1. 重组蛋白
  2. Cytokines and Growth Factors
  3. IGF family
  4. IGFBP-6
  5. IGFBP-6 蛋白, Rat (HEK293, His)

IGFBP-6 蛋白, Rat (HEK293, His)

目录号: HY-P73905
COA 产品使用指南

IGFBP-6 蛋白调节 IGF 的活性,延长其半衰期并表现出双重调节能力。它激活 MAPK 通路,诱导细胞迁移,并与 PHB2 相互作用。这些不同的功能强调了 IGFBP-6 在与生长调节、信号传导和迁移相关的细胞反应中的多方面作用。IGFBP-6 蛋白, Rat (HEK293, His) 是重组的 IGFBP-6 蛋白,由 HEK293 表达,带有 C-His 标签。IGFBP-6 蛋白, Rat (HEK293, His) 全长 201 个氨基酸,分子量约为 25-30 kDa。

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描述

IGFBP-6 protein modulates IGFs' activity, extending their half-life and exhibiting dual regulatory capacity. It activates the MAPK pathway, induces cell migration, and interacts with PHB2. These diverse functions emphasize IGFBP-6's multifaceted role in cellular responses related to growth regulation, signaling, and migration. IGFBP-6 Protein, Rat (HEK293, His) is the recombinant rat-derived IGFBP-6 protein, expressed by HEK293 , with C-His labeled tag. The total length of IGFBP-6 Protein, Rat (HEK293, His) is 201 a.a., with molecular weight of 25-30 kDa.

研究背景

IGFBP-6 蛋白通过延长胰岛素样生长因子 (IGF) 的半衰期,在调节其活性方面发挥着至关重要的作用。IGFBP-6 在细胞培养中表现出双重调节能力,可以抑制或刺激 IGF 的生长促进作用,反映了其对细胞过程的多种影响。除了对 IGF 的影响之外,IGFBP-6 还通过激活 MAPK 通路和促进细胞迁移积极参与细胞信号传导。值得注意的是,它通过其 C 端结构域与 PHB2 相互作用,强调其参与复杂的蛋白质-蛋白质相互作用。这些不同的功能凸显了 IGFBP-6 在协调与生长调节、信号传导和迁移相关的细胞反应中的多方面作用。

生物活性

Measured by its ability to inhibit the biological activity of IGF-I on MCF 7 human breast cancer cells. The ED50 for this effect is 0.1026 µg/mL, corresponding to a specific activity is 9746.5887 U/mg.

种属

Rat

表达系统

HEK293

标签

C-6*His

蛋白编号

P35572/NP_037236.1 (A26-G226)

基因 ID

25641  [NCBI]

蛋白结构
N-term
IGFBP-6 (A26-G226)
Accession # P35572/NP_037236.1
His
C-term
同用名
Insulin-like growth factor-binding protein 6; IBP-6; IGFBP-6; IBP6
氨基酸序列

ALAGCPGCGPGVQEEDAGSPADGCAETGGCFRREGQPCGVYIPKCAPGLQCQPRENEETPLRALLIGQGRCQRARGPSEETTKESKPHGGASRPRDRDRQKNPRTSAAPIRPSPVQDGEMGPCRRHLDSVLQQLQTEVFRGGANGLYVPNCDLRGFYRKQQCRSSQGNRRGPCWCVDPMGQPLPVSPDGQGSSQCSARSSG

分子量

Approximately 25-30 kDa due to the glycosylation

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

IGFBP-6 Protein, Rat (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IGFBP-6 Protein, Rat (HEK293, His)
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