IGFBP-1 蛋白, Rhesus Macaque (HEK293, His)

IGFBP-1 Protein, a member of the insulin-like growth factor-binding protein (IGFBP) family, extends IGFs' half-life and modulates their growth-promoting effects on cell culture, inhibiting or stimulating these responses. Crucially, it alters the interaction between IGFs and their cell surface receptors and promotes cell migration. Notably versatile, IGFBP-1 shows equal binding affinity for both IGF1 and IGF2, underscoring its diverse role in modulating the biological activities of insulin-like growth factors. IGFBP-1 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived IGFBP-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of IGFBP-1 Protein, Rhesus Macaque (HEK293, His) is 234 a.a., with molecular weight of ~30 kDa.

胰岛素样生长因子结合蛋白 1 (IGFBP-1) 是胰岛素样生长因子结合蛋白 (IGFBP) 家族的成员，通过延长 IGF 的半衰期和调节其生长促进作用，有助于调节 IGF 对细胞培养的影响，抑制或刺激这些影响。这种蛋白质在改变 IGF 与其细胞表面受体之间的相互作用方面发挥着至关重要的作用。此外，IGFBP-1 表现出促进细胞迁移的能力，表明它参与生长调节以外的细胞过程。值得注意的是，它对 IGF1 和 IGF2 具有相同的结合亲和力，突出了其在调节胰岛素样生长因子的生物活性中的多功能作用^[1]。

Measured by its binding ability in a functional ELISA. When Recombinant Rhesus IGFBP1 Protein is immobilized at 10 µg/mL (100 µL/well) can bind Biotinylated Recombinant human IGF1. The ED₅₀ for this effect is 81.21 ng/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Rhesus IGFBP1 Protein is immobilized at 10 µg/mL (100 µL/well) can bind Biotinylated Recombinant human IGF1. The ED₅₀ for this effect is 81.21 ng/mL.

Rhesus Macaque

HEK293

C-His

XP_001085935 (A48-N281)

696994  [NCBI]

APWQCAPCSAEKLALCPPVPASCSEVTRSAGCGCCPMCALPLGAACGVATARCARGLSCRALPGEQQPLHALTRGQGACVQDSDASASNAEAAGSPESPESTEITEEELLDNFHLMAPSEEDHSTLWDAIGTYDSSKAVHVTNVKKWKEPCRIELYRVVESLTKAQETSGEDISKFYLPNCNKNGFYHSRQCETSLAGEERLCWCVYPWNGKRIPGSPEIRGDPNCQTYFNVQN

Approximately 30 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.