1. 重组蛋白
  2. Fc Receptors
  3. Immunoglobulin Fc Region
  4. IgG
  5. IgG 蛋白, Rabbit (HEK293)

IgG 蛋白,显示具有 104-Thr 序列的 D12 同种异型标记和具有 185-Thr 序列的 E14 标记。IgG 蛋白, Rabbit (HEK293) 是重组的 IgG 蛋白,由 HEK293 表达,不带标签。

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SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
200 μg ¥3969 1 - 2 周
> 200 μg   询价  

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描述

IgG protein, exhibits the D12 allotypic marker with the 104-Thr sequence and the E14 marker with 185-Thr. IgG Protein, Rabbit (HEK293) is the recombinant rabbit-derived IgG protein, expressed by HEK293, with tag free.

研究背景

IgG 蛋白,显示具有 104-Thr 序列的 D12 同种异型标记和具有 185-Thr 序列的 E14 标记。

种属

Rabbit

表达系统

HEK293

标签

Tag Free

蛋白编号

P01870 (C105-K323)

基因 ID

100009097

蛋白结构
N-term
IgG (C105-K323)
Accession # P01870
C-term
同用名
Ig gamma chain C region; IgG
预测分子量
24.8 kDa.
分子量

Approximately 28-32 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

糖基化修饰
Yes
纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from 0.22 μm filtered solution of 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

IgG Protein, Rabbit (HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IgG Protein, Rabbit (HEK293)
目录号:
HY-P704677
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