1. 重组蛋白
  2. Fc Receptors Biotinylated Proteins
  3. Immunoglobulin Fc Region
  4. IgG1
  5. IgG1 蛋白, Human (Biotinylated, HEK293, Avi)

IgG1 蛋白, Human (Biotinylated, HEK293, Avi)

目录号: HY-P72379
COA 产品使用指南

IgG1蛋白是免疫球蛋白重链的恒定区,在体液免疫识别阶段充当受体。它触发 B 淋巴细胞克隆扩增并分化为分泌免疫球蛋白的浆细胞。IgG1 蛋白, Human (Biotinylated, HEK293, Avi) 是重组的 IgG1 蛋白,由 HEK293 表达,带有 C-Avi 标签。IgG1 蛋白, Human (Biotinylated, HEK293, Avi) 全长 227 个氨基酸,分子量约为 ~33 kDa。

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规格 价格 是否有货 数量
20 μg ¥4250 In-stock
100 μg ¥11500 In-stock
> 100 μg   询价  

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描述

The IgG1 protein is the constant region of the immunoglobulin heavy chain and serves as a receptor during the recognition phase of humoral immunity. It triggers clonal expansion and differentiation of B lymphocytes into immunoglobulin-secreting plasma cells. IgG1 Protein, Human (Biotinylated, HEK293, Avi) is the recombinant human-derived IgG1 protein, expressed by HEK293 , with C-Avi labeled tag. The total length of IgG1 Protein, Human (Biotinylated, HEK293, Avi) is 227 a.a., with molecular weight of ~33 kDa.

研究背景

免疫球蛋白重链的恒定区(称为抗体)代表 B 淋巴细胞产生的膜结合或分泌糖蛋白。在体液免疫的识别阶段,这些膜结合的免疫球蛋白充当受体,在结合特定抗原后启动B淋巴细胞的克隆扩增和分化为分泌免疫球蛋白的浆细胞。分泌的免疫球蛋白在体液免疫的效应阶段发挥着至关重要的作用,导致结合抗原的消除。抗原结合位点由一条重链的可变结构域及其相关轻链的可变结构域形成,导致每种免疫球蛋白具有两个对特定抗原具有显着亲和力的抗原结合位点。可变结构域经历 V-(D)-J 重排和随后的体细胞超突变,从而在暴露和选择后实现特定抗原的亲和力成熟。IgG1 蛋白介导单核细胞的效应功能,触发病毒感染细胞的抗体依赖性细胞毒性 (ADCC)。免疫球蛋白由两条相同的重链和两条相同的轻链组成,通过二硫键互连,并与 FCGR1A、FCGR2A 和 FCGR3A 相互作用以介导各种效应功能。

种属

Human

表达系统

HEK293

标签

C-Avi

蛋白编号

P01857 (D104-K330, D239E, L241M)

基因 ID
蛋白结构
N-term
IgG1 (D104-K330, D239E, L241E)
Accession # P01857
Avi
C-term
同用名
Ig gamma-1 chain C region; IGHG1
氨基酸序列

DKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCEVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK

分子量

Approximately 33 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

IgG1 Protein, Human (Biotinylated, HEK293, Avi) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IgG1 Protein, Human (Biotinylated, HEK293, Avi)
目录号:
HY-P72379
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