IL-1 alpha/IL-1F1 蛋白, Porcine

The IL-1α/IL-1F1 protein is found intracellularly in most non-hematopoietic cells and plays a crucial role in mediating inflammation and linking innate and adaptive immunity. IL1RAP binds to IL1R1 to form a high-affinity receptor complex that activates cascades and pathways. IL-1 alpha/IL-1F1 Protein, Porcine is the recombinant Porcine-derived IL-1 alpha/IL-1F1 protein, expressed by E. coli , with tag free. The total length of IL-1 alpha/IL-1F1 Protein, Porcine is 152 a.a., with molecular weight of ~17.5 kDa.

细胞因子白介素 1 α (IL-1 α) 基本存在于几乎所有静止非造血细胞的细胞内，在炎症中发挥着至关重要的作用，并作为连接先天性免疫系统和适应性免疫系统的关键介质。IL-1α 与其受体 IL1R1 及其辅助蛋白 IL1RAP 结合后，形成高亲和力白细胞介素 1 受体复合物，启动涉及招募接头分子（如 MYD88、IRAK1 或 IRAK4）的信号级联反应。这种激活导致 NF-kappa-B 和三个 MAPK 途径（p38、p42/p44 和 JNK 途径）的后续激活。在细胞内，IL-1 α 作为警报素，在细胞死亡时、细胞膜破裂后释放到细胞外空间，诱导炎症并向宿主发出对损伤或损害的反应信号。除了在细胞坏死期间充当危险信号外，IL-1 α 还可以直接感知 DNA 损伤，作为基因毒性应激的信号，而不会损害细胞完整性。作为单体，IL-1 α 与 TMED10 相互作用，促进从细胞质易位至内质网-高尔基中间室 (ERGIC) 以及随后的分泌。此外，IL-1α与IL1R1和S100A13相互作用，后者相互作用是IL-1α输出的初始步骤，随后该复合物直接易位穿过质膜。

1.Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED₅₀ for this effect is 10-60 pg/mL.
2.Measured in a cell proliferation assay using CTTL2 cells. The ED₅₀ for this effect is 6.554 pg/mL, corresponding to a specific activity is 1.526×10⁸ units/mg.

Porcine

E. coli

Tag Free

P18430 (Q119-S270)

397094  [NCBI]

QSNMKYNFMRVINHQCILNDARNQSIIRDPSGQYLMAAVLNNLDEAVKFDMAAYTSNDDSQLPVTLRISETRLFVSAQNEDEPVLLKELPETPKTIKDETSLLFFWEKHGNMDYFKSAAHPKLFIATRQEKLVHMAPGLPSVTDFQILENQS

Approximately 17.5 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS and DTT with BSA as a carrier protein.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.