1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-10
  5. IL-10 蛋白, Human (CHO)

IL-10 蛋白, Human (CHO)

目录号: HY-P7030A
COA 产品使用指南

IL-10 蛋白, Human (CHO)是一种 CHO 细胞来源的调节粘膜炎症的抗炎免疫调节细胞因子。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
Free Sample   Apply now  
2 μg ¥570 In-stock
10 μg ¥1600 In-stock
50 μg ¥5600 In-stock
100 μg ¥9500 In-stock
> 100 μg   询价  

* Please select Quantity before adding items.

注册 MCE会员完成审核
即刻享有 积分商城 300 专属积分

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的科研文献

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

IL-10 Protein, Human (CHO) is a CHO cell derived anti-inflammatory, immunomodulatory cytokine that regulates mucosal inflammation.

研究背景

白细胞介素 10 (IL-10) 是一种抗炎、免疫调节细胞因子,可调节粘膜炎症。一种有前途的替代品是白细胞介素 10 (IL-10),这是一种具有多种抗炎和免疫调节活性的细胞因子,包括抑制 T 细胞/巨噬细胞活化和促炎细胞因子合成[1]IL-10 是一种具有强抗炎特性的细胞因子,目前正在研究其作为免疫抑制剂的治疗用途。IL-10 和皮质类固醇的免疫抑制作用包括在蛋白质和信使 RNA 水平上抑制单核细胞和多形核白细胞(IFN-γ、TNF-α、IL-1β、IL-6、GM-CSF)产生促炎细胞因子,以及预防有丝分裂原诱导的 T 细胞增殖[2]

生物活性

1.The ED50 is <0.2 ng/mL as measured by MC/9 cells.
2.Immobilized human IL10 at 10 μg/mL (100 μl/well) can bind Cynomolgus IL10RA. The ED50 for this effect is 1.732 μg/mL.

  • Immobilized human IL10 at 10 μg/mL (100 μL/well) can bind Cynomolgus IL10RA,The ED50 for this effect is 1.732 μg/mL.
种属

Human

表达系统

CHO

标签

Tag Free

蛋白编号

P22301 (S19-N178)

基因 ID
蛋白结构
N-term
IL-10 (S19-N178)
Accession # P22301
C-term
同用名
rHuIL-10; CSIF
氨基酸序列

SPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCHRFLPCENKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRN

分子量

18-19 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized after extensive dialysis against PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

IL-10 Protein, Human (CHO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
IL-10 Protein, Human (CHO)
目录号:
HY-P7030A
需求量: