IL-10 蛋白, Human

IL-10 Protein, Human is an anti-inflammatory, immunomodulatory cytokine that regulates mucosal inflammation.

白细胞介素 10 (IL-10) 是一种抗炎、免疫调节细胞因子，可调节粘膜炎症。一种有前途的替代品是白细胞介素 10 (IL-10)，这是一种具有多种抗炎和免疫调节活性的细胞因子，包括抑制 T 细胞/巨噬细胞活化和促炎细胞因子合成^[1]。IL-10 是一种具有强抗炎特性的细胞因子，目前正在研究其作为免疫抑制剂的治疗用途。IL-10 和皮质类固醇的免疫抑制作用包括在蛋白质和信使 RNA 水平上抑制单核细胞和多形核白细胞（IFN-γ、TNF-α、IL-1β、IL-6、GM-CSF）产生促炎细胞因子，以及预防有丝分裂原诱导的 T 细胞增殖^[2]。

1.The ED₅₀ is typically 1- 8 ng/mL as measured by MC/9-2 mouse mast cells in a cell proliferation assay.
2.Immobilized human IL10 at 10 μg/mL (100 μL/well) can bind Cynomolgus IL10RA-Fc and the EC₅₀ of Cynomolgus IL10RA-Fc is 0.24-0.56 μg/mL.
3.Immobilized human IL10 at 2 μg/mL (100 μL/well) can bind Cynomolgus IL10RA-Fc and the EC₅₀ of Cynomolgus IL10RA-Fc is 0.1-0.3 μg/mL.

Human

E. coli

Tag Free

P22301 (S19-N178)

3586  [NCBI]

SPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCHRFLPCENKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRN

Approximately 18.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against 20mM Tris, 20mM NaCl, pH 6.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Fedorak RN, et al. Recombinant human interleukin 10 in the treatment of patients with mild to moderately active Crohn's disease. The Interleukin 10 Inflammatory Bowel Disease Cooperative Study Group. Gastroenterology. 2000 Dec;119(6):1473-82.  [Content Brief]

  [2]. Chakraborty A, et al. Pharmacodynamic interaction of recombinant human interleukin-10 and prednisolone using in vitro whole blood lymphocyte proliferation. J Pharm Sci. 2002 May;91(5):1334-42.  [Content Brief]