1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-12
  5. IL-12 beta
  6. IL-12 beta 蛋白, Mouse (HEK293)

IL-12 beta 蛋白, Mouse (HEK293)

目录号: HY-P73161
产品使用指南

IL-12 beta 蛋白,也被称为自然杀伤细胞刺激因子 2,是 IL-12 和 IL-23 的共有亚单位 (p40)。IL-12 是一种由活化的巨噬细胞表达的炎症因子,参与对癌症的 Th1 型免疫应答。IL-12 beta 蛋白位于细胞膜外,参与 Jak-STAT 介导的信号传导,由 335 个氨基酸 (M1-S335) 组成,具有纤维连接蛋白 III 型结构域 (233-324 a.a)。小鼠重组 IL-12 beta 蛋白 (M1-S335) 在 HEK293 细胞中产生,不带有标签。

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描述

IL-12 beta Protein, also known as natural killer cell stimulatory factor 2, is a common subunit (p40) of IL-12 and IL-23. IL-12 is a inflammatory factor expressed by activated macrophages, and involves in Th1-type immune response against cancer[1]. IL-12 beta Protein located outside the cell membrane, involves in singalling mediated by Jak-STAT[3]. IL-12 beta Protein consists of 335 amino acids (M1-S335) with a fibronectin type-III domain (233-324 a.a). IL-12 beta Protein, Mouse (M1-S335) is produced in HEK293 cells with tag free.

研究背景

IL-12 beta Protein, as known as IL12 p40 subunit or IL-12B, heterodimerizes with the IL-12 p35 subunit (IL-12A) to form IL-12 and with the IL23 p19 subunit to form IL-23, exerting different regulating functions[1].
IL-12 and IL23 belong to IL-12 family, are involved in proinflammatory responses and expressed by activated macrophages that serve as an essential inducer of Th1 cells development[2].
IL-12 signals via IL-12Rβ1 and IL-12Rβ2 mediated by p-STAT4, whereas IL-23 signals via IL-12Rβ1 and IL-23R mediated by p-STAT1 and p-STAT3[3].
The sequence of amino acids in IL-12 beta proteins of mouse is very different from human (69.04%), but shows high similarity with rat (92.24%).
Interleukin 12 (IL-12) family have been known to be inflammatory factors, induce autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis[4].

体外研究
(In Vitro)

Mouse IL-12 (mIL-12) has a significant stimulating effect on CTLL-2 cells and results 65-70% of CTLL2 cell population having mIL12 receptors[5].
Mouse IL-12 (mIL-12) (0.19-50 U/mL; 24-72 h) inhibits CTLL-2 cells proliferation without cell-lethal effect at 2 U/mL for 72 h[5].

体内研究
(In Vivo)

IL12b-/- (p40-deficient) mice exhibits decreasing blood neutrophil numbers and an absence of serum levels of IL-17A[6].
  IL12b-/-/Itgb2-/- (lacking adhesion molecules like beta2-integrins) mice has a reducing tissue mRNA expression of IL-17A compared with Itgb2-/- controls and reduces total number of CD3+ IL-17A-producing Tn cells in the spleen and lamina propria[6].
IL12b contributes to Aβ42-induced neuronal damage and cognitive decline in APPPS1 Alzheimer's disease mouse model[7].

生物活性

Measured by its binding ability in a functional ELISA. Immobilized mouse IL12B at 10μg/mL(100μL/well) can bind human IL12RB1-His and the EC50 is 0.11-0.25μg/mL.

种属

Mouse

表达系统

HEK293

标签

Tag Free

蛋白编号

P43432 (M1-S335)

基因 ID

16160  [NCBI]

中文名
重组小鼠 IL-12 beta 蛋白 (HEK293)
同用名
Interleukin-12 subunit beta; IL-12B; CLMF p40; IL-12 subunit p40; NKSF2; IL12B
分子量

Approximately 46 kDa

纯度

Greater than 85% as determined by reducing SDS-PAGE

性状

Lyophilized powder.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

IL-12 beta Protein, Mouse (HEK293) 相关分类

  • 复溶计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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