1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-12
  5. IL-12 alpha IL-12 beta
  6. IL-12 蛋白, Mouse (HEK293, Fc)

IL-12 蛋白, Mouse (HEK293, Fc)

目录号: HY-P73157
产品使用指南

Interleukin-12 subunit alpha (IL-12A; IL-12p35) 是一种免疫抑制细胞因子,编码细胞因子 IL-12 的亚基,作用于 T 细胞和自然杀伤细胞,并具有广泛的生物活性。IL-12A 与IL-12B 异二聚化形成 IL-12 细胞因子或与 EBI3/IL27B 形成 IL-35 细胞因子。IL-12 蛋白, Mouse (HEK293, Fc) 是在 HEK293 细胞中产生的,由 IL-12A (M1-A215) 和 IL-12B (M1-S335) 组成,其 C 末端带有Fc 标签。

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描述

Interleukin-12 subunit alpha (IL-12A; IL-12p35), an immune-suppressive cytokine, encodes a subunit of the cytokine IL-12 that acts on T and natural killer cells, and has a broad array of biological activities. IL-12A heterodimerizes with IL-12B to form the IL-12 cytokine or with EBI3/IL27B to form the IL-35 cytokine[1][2]. IL-12 Protein, Mouse (HEK293, Fc) is produced in HEK293 cells with a C-Terminal Fc-tag. It consists of IL-12A (M1-A215) and IL-12B (M1-S335).

研究背景

Interleukin-12 subunit alpha (IL-12A; IL-12p35), an immune-suppressive cytokine, encodes a subunit of the cytokine IL-12 that acts on T and natural killer cells, and has a broad array of biological activities. IL-12A is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. IL-12 is primarily produced by professional antigen-presenting cells (APCs) such as B-cells and dendritic cells (DCs) as well as macrophages and granulocytes, induces the production of IFN-gamma, favors the differentiation of Th1 cells and is an important link between innate resistance and adaptive immunity[1][2].
The amino acid sequence of human IL-12A protein has low homology between mouse IL-12A protein. While, human IL-12A shares 94% aa sequence identity with Rhesus Macaque IL-12A protein.
IL-12 family cytokines are pleiotropic immunological playmakers that coordinate innate and adaptive immune responses mainly via regulation of T-cell populations. The four core members of the Interleukin-12 (IL-12) family of cytokines, IL-12, IL-23, IL-27 and IL-35 are heterodimers which share α-cytokine subunits (IL-12p35 (IL-12A), IL-23p19, and IL-27p28) and β-cytokine subunits (IL-12p40, Ebi3). The subunits are each encoded by separate chromosomes and their expression is regulated independently. Among them, the IL-12A subunit has immunoregulatory functions hitherto attributed to IL-35. Pairing of the α-subunits, IL-12A or IL-23p19 with IL-12p40, gives rise to the two pro-inflammatory members IL-12 and IL-23, respectively, whereas the two immunosuppressive members of the family, IL-27 and IL-35, derive from pairing of IL-27p28 or IL-12A with Ebi3[1][2].
IL-12A suppresses lymphocyte proliferation, induces expansion of IL-10-expressing and IL-35-expressing B cells and ameliorates autoimmune uveitis in mice by antagonizing pathogenic Th17 responses. IL-12A-mediated expansion of Treg and Breg cells and its amelioration of experimental autoimmune encephalomyelitis (EAE) correlated with inhibition of cytokine-induced activation of STAT1/STAT3 pathways. IL-12A may be utilized for in vivo expansion of Tregs and Bregs cells and autologous Tregs and Bregs cell immunotherapy[1][2].

体外研究
(In Vitro)

IL-12 alpha Protein, Mouse (6.25-100 ng/mL; 72 hours) suppresses T cell and B cell proliferation in dose-dependent manner[1].
IL-12 alpha Protein, Mouse (100 ng/mL; 1 hour) inhibits of cytokine-induced activation of STAT1/STAT3 pathways in CD4+ T cells. It also inhibits lymphocyte proliferation by suppressing the expressions of cell-cycle regulatory proteins in CD19+ B cells[2].

体内研究
(In Vivo)

IL-12 alpha Protein, Mouse (100 ng/mouse; intraperitoneal injection; every other day; for 12 days) inhibits the expansion of pathogenic Th17 and Th1 cells and inhibits trafficking of inflammatory cells into the brain and spinal cord in mouse model of experimental autoimmune encephalomyelitis (EAE)[2].

生物活性

Immobilized mouse mIL12A+mIL12B at 10 μg/mL (100 μl/well) can bind human IL12RB1-His and the EC50 is 38.7-90.3 ng/mL. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells and the ED50  is 0.1-0.8 μg/mL.

种属

Mouse

表达系统

HEK293

标签

C-hFc

蛋白编号

P43431 (M1-A215)&P43432 (M1-S335)

基因 ID

16159  [NCBI]&16160  [NCBI]

中文名
重组小鼠白细胞介素-12 (HEK293, Fc)
同用名
IL-12; Interleukin 12; Interleukin-12 subunit alpha; IL-12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; Interleukin-12 subunit beta; IL-12B; Cytotoxic lymphocyte maturation factor 40 kDa subunit; CLMF p40; IL-12 subunit p40
分子量

Approximately 55&75 kDa

纯度

Greater than 85% as determined by reducing SDS-PAGE

性状

Lyophilized powder.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IL-12 Protein, Mouse (HEK293, Fc)
目录号:
HY-P73157
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