1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-12
  5. IL-12 alpha IL-12 beta
  6. IL-12 蛋白, Mouse (HEK293, His)

IL-12 蛋白, Mouse (HEK293, His)

目录号: HY-P73159
COA 产品使用指南

Interleukin-12 subunit alpha (IL-12A; IL-12p35) 是一种免疫抑制细胞因子,编码细胞因子 IL-12 的亚基,作用于 T 细胞和自然杀伤细胞,并具有广泛的生物活性。IL-12A 与IL-12B 异二聚化形成 IL-12 细胞因子或与 EBI3/IL27B 形成 IL-35 细胞因子。IL-12 蛋白, Mouse (HEK293, His) 是在 HEK293 细胞中产生的,由 IL-12A (M1-A215) 和 IL-12B (M1-S335) 组成,其 C 末端带有His 标签。

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规格 价格 是否有货 数量
50 μg ¥4000 In-stock
100 μg   询价  

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描述

Interleukin-12 subunit alpha (IL-12A; IL-12p35), an immune-suppressive cytokine, encodes a subunit of the cytokine IL-12 that acts on T and natural killer cells, and has a broad array of biological activities. IL-12A heterodimerizes with IL-12B to form the IL-12 cytokine or with EBI3/IL27B to form the IL-35 cytokine[1][2]. IL-12 Protein, Mouse (HEK293, His) is produced in HEK293 cells with a C-Terminal His-tag. It consists of IL-12A (M1-A215) and IL-12B (M1-S335).

研究背景

Interleukin-12 α 亚基(IL-12A;IL-12p35)是一种免疫抑制细胞因子,编码细胞因子 IL-12 的一个亚基,作用于 T 细胞和自然杀伤细胞,并具有广泛的生物活性。IL-12A 是一种二硫键连接的异二聚体,由该基因编码的 35 kD 亚基和属于细胞因子受体家族成员的 40 kD 亚基组成。IL-12 主要由 B 细胞和树突状细胞 (DC) 等专业抗原呈递细胞 (APC) 以及巨噬细胞和粒细胞产生,诱导 IFN-γ 的产生,有利于 Th1 细胞的分化,是一种先天抵抗和适应性免疫之间的重要联系[1][2]
人IL-12A蛋白的氨基酸序列与小鼠IL-12A蛋白的氨基酸序列具有较低的同源性12A蛋白。而人 IL-12A 与恒河猴 IL-12A 蛋白具有 94% 的氨基酸序列同一性。
IL-12 家族细胞因子是多效性免疫游戏制造者,主要通过调节 T 细胞群来协调先天性和适应性免疫反应。IL-12 (IL-12) 细胞因子家族的四个核心成员 IL-12、IL-23、IL-27 和 IL-35 是共享 α-细胞因子亚基的异二聚体 (IL-12p35 (IL-12A) 、IL-23p19 和 IL-27p28)和 β-细胞因子亚基(IL-12p40、Ebi3)。每个亚基都由单独的染色体编码,并且它们的表达受到独立调节。其中,IL-12A亚基具有迄今为止归因于IL-35的免疫调节功能。α 亚基 IL-12A 或 IL-23p19 与 IL-12p40 配对,分别产生两个促炎成员 IL-12IL-23,而该家族的两个免疫抑制成员 IL-27和 IL-35,源自 IL-27p28 或 IL-12A 与 Ebi3[1][2] 配对。
IL-12A 抑制淋巴细胞增殖,诱导表达 IL-10 和表达 IL-35 的 B 细胞扩增,并通过拮抗致病性 Th17 反应来改善小鼠自身免疫性葡萄膜炎。IL-12A 介导的 Treg 和 Breg 细胞扩增及其对实验性自身免疫性脑脊髓炎 (EAE) 的改善与抑制细胞因子诱导的 STAT1/STAT3 通路激活相关。IL-12A 可用于 Tregs 和 Breg 细胞的体内扩增以及自体 Tregs 和 Breg 细胞免疫治疗[1][2]

体外研究
(In Vitro)

IL-12 alpha Protein, Mouse (6.25-1 ng/mL; 72 hours) suppresses T cell and B cell proliferation in dose-dependent manner[1].
IL-12 alpha Protein, Mouse (1 ng/mL; 1 hour) inhibits of cytokine-induced activation of STAT1/STAT3 pathways in CD4+ T cells. It also inhibits lymphocyte proliferation by suppressing the expressions of cell-cycle regulatory proteins in CD19+ B cells[2].

体内研究
(In Vivo)

IL-12 alpha Protein, Mouse (1 ng/mouse; intraperitoneal injection; every other day; for 12 days) inhibits the expansion of pathogenic Th17 and Th1 cells and inhibits trafficking of inflammatory cells into the brain and spinal cord in mouse model of experimental autoimmune encephalomyelitis (EAE)[2].

生物活性

1.Measured by its ability to bind recombinant mouse IL12RB2 in a functional ELISA.
2.Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells and the ED50 is 1-10 ng/mL.

种属

Mouse

表达系统

HEK293

标签

C-6*His

蛋白编号

P43431 (R23-A215) & P43432 (M23-S335)

基因 ID
中文名
重组小鼠白细胞介素-12 (HEK293, His)
同用名
IL-12; Interleukin 12; Interleukin-12 subunit alpha; IL-12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; Interleukin-12 subunit beta; IL-12B; Cytotoxic lymphocyte maturation factor 40 kDa subunit; CLMF p40; IL-12 subunit p40
分子量

Approximately 28&47 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IL-12 Protein, Mouse (HEK293, His)
目录号:
HY-P73159
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