1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-13
  5. IL-13 蛋白, Mouse (CHO, His)

IL-13 蛋白, Mouse (CHO, His)

目录号: HY-P7076A
COA 产品使用指南

IL-13 蛋白, Mouse (CHO, His)是一种 CHO 细胞来源的重要细胞因子,是一种多功能细胞因子,其主要作用是减少炎症反应。

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规格 价格 是否有货 数量
10 μg ¥2400 In-stock
50 μg ¥8400 In-stock
100 μg   询价  

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  • 生物活性

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  • 产品资料

  • 参考文献

描述

IL-13 Protein, Mouse (CHO, His), an important CHO cell derived cytokine, is a multifunctional cytokine whose principal action is to diminish inflammatory responses.

研究背景

白细胞介素(IL)-13在过敏性哮喘的发病机制中发挥着关键作用。在动物模型中,被动施用其单克隆抗体或可溶性受体来阻断过量产生的IL-13已被证明可以有效控制气道过敏反应,但这些方法存在半衰期短、成本高和可能产生不良反应的缺点。IL-13诱导Ca2+激活氯通道的基因表达,刺激上皮细胞分化,促进粘蛋白基因表达和释放。IL-13 在哮喘发病机制中发挥着关键作用[1]

生物活性

The ED50 is <20 ng/mL as measured by human TF-1 cells.

种属

Mouse

表达系统

CHO

标签

C-6*His

蛋白编号

P20109 (P22-F131)

基因 ID
蛋白结构
N-term
IL-13 (P22-F131)
Accession # P20109
6*His
C-term
同用名
rMuIL-13, His; P600
氨基酸序列

PVPRSVSLPLTLKELIEELSNITQDQTPLCNGSMVWSVDLAAGGFCVALDSLTNISNCNAIYRTQRILHGLCNRKAPTTVSSLPDTKIEVAHFITKLLSYTKQLFRHGPFHHHHHH

分子量

14-30 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized after extensive dialysis against PBS.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

IL-13 Protein, Mouse (CHO, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IL-13 Protein, Mouse (CHO, His)
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