1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-13
  5. IL-13 蛋白, Mouse (HEK293)

IL-13 蛋白, Mouse (HEK293)

目录号: HY-P73168
COA 产品使用指南

IL-13 蛋白是一种细胞因子,参与过敏性炎症、寄生虫免疫反应和 B 细胞增殖。IL-13 蛋白, Mouse (HEK293) 是重组的 IL-13 蛋白,由 HEK293 表达,不带标签。IL-13 蛋白, Mouse (HEK293) 全长 113 个氨基酸,分子量约为 ~14 kDa。

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规格 价格 是否有货 数量
20 μg ¥2350 In-stock
100 μg ¥6500 In-stock
> 100 μg   询价  

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描述

IL-13 protein is a cytokine involved in allergic inflammation, immune response to parasites, and B cell proliferation. IL-13 Protein, Mouse (HEK293) is the recombinant mouse-derived IL-13 protein, expressed by HEK293 , with tag free. The total length of IL-13 Protein, Mouse (HEK293) is 113 a.a., with molecular weight of ~14 kDa.

研究背景

IL-13 蛋白在过敏性炎症和寄生虫感染的免疫反应中发挥着关键作用。它与 IL2 协同调节干扰素-γ 合成、刺激 B 细胞增殖以及激活嗜酸性粒细胞、嗜碱性粒细胞和肥大细胞。除了其免疫功能外,IL-13 通过调节跨膜和可溶形式的 IL-1 受体样 1/IL1RL1 的产生,在控制 IL33 活性方面发挥着至关重要的作用。它展示了拮抗 Th1 驱动的促炎性免疫反应并下调各种促炎细胞因子(包括 IL1、IL6、IL10、IL12 和 TNF-α)合成的能力,部分通过抑制 NF-kappa-B 实现。IL-13 不仅作用于造血细胞,还作用于内皮细胞等非造血细胞,诱导血管细胞粘附蛋白 1/VCAM1,这对于嗜酸性粒细胞的募集至关重要。其生物学效应是通过包含IL4R链和IL13RA1链的受体介导的,激活JAK1和TYK2,最终导致STAT6的激活。除了 IL13RA1 之外,另一种受体 IL13RA2 可作为 IL-13 的高亲和力诱饵,介导细胞外 IL-13 的内化和消耗。IL-13 直接与 IL13RA2 相互作用,进一步说明其调节机制的复杂性。

生物活性

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells and the ED50 is typically 2-12 ng/mL.

种属

Mouse

表达系统

HEK293

标签

Tag Free

蛋白编号

P20109 (A19-F131)

基因 ID
蛋白结构
N-term
IL-13 (A19-F131)
Accession # P20109
C-term
同用名
IL13; Interleukin-13; IL-13; NC30
分子量

Approximately 14 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

IL-13 Protein, Mouse (HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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