IL-15 蛋白, Human

IL-15 Protein, Human is a pleiotropic cytokine, including T, B and NK cell-stimulatory activities.

白介素 15 是一种细胞因子，与 IL-2 具有许多相同的生物学特性，包括 T、B 和 NK 细胞刺激活性。Interleukin-15 作为抗肿瘤 CD8 T 和自然杀伤 (NK) 细胞的激活剂，在癌症免疫治疗中具有巨大潜力^[1]。白介素 15 (IL-15) 是一种多效性细胞因子，是细胞因子四个 α 螺旋束家族的成员，其中包括 IL-2、IL-4、IL-7、IL-9、IL-15 和 IL-21 。IL-15 具有广泛的生物活性，可诱导 T、B 和自然杀伤 (NK) 细胞的分化和增殖。作为一种有效的促炎细胞因子，IL-15 在自身免疫性疾病和炎症中发挥着重要而复杂的作用。IL-15在一些血液恶性肿瘤中发挥着关键作用并具有抗肿瘤作用。IL-15 的直接给药已在多种临床前小鼠肿瘤模型中显示出抗肿瘤作用^[2]。

1. The ED₅₀ is <0.5 ng/mL as measured by CTLL-2 cells, corresponding to a specific activity of >2 × 10⁶ units/mg.
2.The ED₅₀ is less than 2 ng/mL, as measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells.

• 
  The ED₅₀ is 2 ng/mL, as measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells.

Human

E. coli

Tag Free

P40933 (N49-S162)

3600  [NCBI]

NWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISHESGDTDIHDTVENLIILANNILSSNGNITESGCKECEELEEKNIKEFLQSFVHIVQMFINTS

Approximately 12.8 kD

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against PBS or 20 mM PB, 150 mM NaCl, pH 7.0 or PBS.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Conlon KC, et al. Redistribution, hyperproliferation, activation of natural killer cells and CD8 T cells, and cytokine production during first-in-human clinical trial of recombinant human interleukin-15 in patients with cancer. J Clin Oncol. 2015 Jan 1;33(1):74-82.  [Content Brief]

  [2]. Sun W, et al. High level expression and purification of active recombinant human interleukin-15 in Pichiapastoris. J Immunol Methods. 2016 Jan;428:50-7.  [Content Brief]