1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-15 IL-15 Receptor
  5. IL-15R alpha
  6. IL-15R alpha & IL-15 Fusion 蛋白, Human (HEK293, Fc)

IL-15R alpha & IL-15 Fusion 蛋白, Human (HEK293, Fc)

目录号: HY-P70655
COA 产品使用指南

IL-15R alpha 是 IL-15 的高亲和力受体 (Kd: 100 pM)。IL-15 对于维持 NK 细胞, NKT 细胞 和 记忆 CD8+ T 细胞的功能和发展起着重要的作用。IL-15R alpha 维持着记忆 CD8 T 细胞的稳态和淋巴细胞的发育。IL-15R alpha/IL-15 复合体 可激活 NK 细胞和 CD8+ T 细胞,使其具备抗肿瘤能力。IL-15R alpha 蛋白, Human (142a.a, HEK293, Fc) 是一种由 HEK293 细胞产生的重组人 IL-15R alpha (I31-T172) & IL-15 (N49-S162) 融合蛋白,其 C 末端带有 hFc 标签。

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规格 价格 是否有货 数量
10 μg ¥1200 In-stock
50 μg ¥3600 询价
100 μg   询价  
250 μg   询价  

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描述

IL-15R alpha is a high affinity receptor for IL-15 (Kd: 100 pM). IL-15 is essential for the development and function natural killer (NK) cell, NKT cell and memory (m) CD8+ T cell[1]. IL-15R alpha maintains memory CD8+ T cell homeostasis and lymphocyte development. IL-15R alpha/IL-15 complex can activate the antitumor functions of NK cells and CD8+ T cells[2][3]. IL-15R alpha & IL-15 Fusion Protein, Human (HEK293, Fc) is a recombinant human IL-15R alpha (I31-D96) & IL-15 (N49-S162) fusion protein with a C-Terminal hFc tag, which is produced in HEK293 cells.

研究背景

IL-15R alpha is expressed on various cell types, including lymphocytes, myeloid cells, nonlymphoid and nonhematopoietic cells[4]. IL-15 is produced in macrophages and dendritic cells[1].
IL-15R alpha is required for transporting of IL-15 from the endoplasmic reticulum to the cell surface to bind with β (CD122) and γ (CD132) chains on responding lymphocytes[4][5]. When binding with IL-15, the complex increases the in vivo half-life of IL-15 and enhances binding affinity of IL-15 with IL-15Rβ/γ in NK cells and CD8+ T cells. Thus, the signal transmission improves proliferation and antitumor activities of NK cells and CD8+ T cells[2].
IL-15R alpha forms complex with IL-15 and activates the antitumor functions of NK cells and CD8+ T cells. IL-15R alpha/IL-15 complex is a potential immunotherapeutic agent for cancer and viral infection[1].

体内研究
(In Vivo)

IL-15R alpha (mouse, pre-complexed with hIL-15, i.p., 15 μg, 200 μL) significantly reduces the tumor volume, and induces an early increase of activated NK cells in B16-F10 melanoma mice[6].
IL-15R alpha & IL-15 Fusion Protein (mouse, i.p., 15 μg & 2.5 μg, 200 μL) causes naive CD8 T cell activation and development into effector cells and long-term memory T cells[7].

生物活性

The cell proliferation assay using CTLL-2 mouse cytotoxic T cells has an ED50 value of 5-20 ng/mL.

种属

Human

表达系统

HEK293

标签

C-hFc

蛋白编号

Q13261 (I31-D96)&P40933 (N49-S162, N120D)

基因 ID

3601  [NCBI]&3600  [NCBI]

中文名
重组人 (HEK293, Fc) 白介素-15 受体 alpha & 白介素-15融合蛋白
同用名
IL15RA& IL15; Interleukin-15; IL-15; IL15; IL-15 receptor subunit alpha; IL-15RA; IL-15R-alpha; interleukin-15 receptor subunit alpha
氨基酸序列

ITCPPPMSVEHADIWVKSYSLYSRERYICNSGFKRKAGTSSLTECVLNKATNVAHWTTPSLKCIRD
&:
NWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISLESGDASIHDTVENLIILANDSLSSNGNVTESGCKECEELEEKNIKEFLQSFVHIVQMFINTS

分子量

50-60 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

Lyophilized powder.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, 5% Trehalose, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料
参考文献

IL-15R alpha & IL-15 Fusion Protein, Human (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IL-15R alpha & IL-15 Fusion Protein, Human (HEK293, Fc)
目录号:
HY-P70655
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