1. 重组蛋白
  2. Cytokines and Growth Factors Biotinylated Proteins
  3. Interleukin & Receptors
  4. IL-15 IL-15 Receptor
  5. IL-15R alpha
  6. IL-15R alpha & IL-15 蛋白, Human (Biotinylated, HEK293, His)

IL-15R alpha & IL-15 蛋白, Human (Biotinylated, HEK293, His)

目录号: HY-P77700
产品使用指南

IL-15R alpha 是 IL-15 的高亲和力受体 (Kd: 100 pM)。IL-15 对于维持 NK 细胞, NKT 细胞 和 记忆 CD8+ T 细胞的功能和发展起着重要的作用。IL-15R alpha 维持着记忆 CD8 T 细胞的稳态和淋巴细胞的发育。IL-15R alpha/IL-15 复合体 可激活 NK 细胞和 CD8+ T 细胞,使其具备抗肿瘤能力。IL-15R alpha & IL-15 蛋白, Human (Biotinylated, HEK293, His) 是一种由 HEK293 细胞产生的生物素化的重组人 IL-15R alpha (I31-S108) & IL-15 (N49-S162) 融合蛋白,其 C 末端带有 His 标签。

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100 μg 询问价格 & 货期

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描述

IL-15R alpha is a high affinity receptor for IL-15 (Kd: 100 pM). IL-15 is essential for the development and function natural killer (NK) cell, NKT cell and memory (m) CD8+ T cell[1]. IL-15R alpha maintains memory CD8+ T cell homeostasis and lymphocyte development. IL-15R alpha/IL-15 complex can activate the antitumor functions of NK cells and CD8+ T cells[2][3]. IL-15R alpha & IL-15 Protein, Human (Biotinylated, HEK293, His) is a recombinant biotinylated human IL-15R alpha (I31-S108) & IL-15 (N49-S162) fusion protein with a C-Terminal His tag, which is produced in HEK293 cells.

研究背景

IL-15R alpha is expressed on various cell types, including lymphocytes, myeloid cells, nonlymphoid and nonhematopoietic cells[4]. IL-15 is produced in macrophages and dendritic cells[1].
IL-15R alpha is required for transporting of IL-15 from the endoplasmic reticulum to the cell surface to bind with β (CD122) and γ (CD132) chains on responding lymphocytes[4][5]. When binding with IL-15, the complex increases the in vivo half-life of IL-15 and enhances binding affinity of IL-15 with IL-15Rβ/γ in NK cells and CD8+ T cells. Thus, the signal transmission improves proliferation and antitumor activities of NK cells and CD8+ T cells[2].
IL-15R alpha forms complex with IL-15 and activates the antitumor functions of NK cells and CD8+ T cells. IL-15R alpha/IL-15 complex is a potential immunotherapeutic agent for cancer and viral infection[1].

体内研究
(In Vivo)

IL-15R alpha (mouse, pre-complexed with hIL-15, i.p., 15 μg, 200 μL) significantly reduces the tumor volume, and induces an early increase of activated NK cells in B16-F10 melanoma mice[6].
IL-15R alpha & IL-15 Fusion Protein (mouse, i.p., 15 μg & 2.5 μg, 200 μL) causes naive CD8 T cell activation and development into effector cells and long-term memory T cells[7].

生物活性

Immobilized Human IL-2 R beta&IL-2 R gamma at 1 μg/mL (100μL/Well) on the plate. Dose response curve for Biotinylated Human IL-15RA&IL-15 with the EC50 of 13.2 ng/mL determined by ELISA.

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

Q13261 (I31-S108)&P40933 (N49-S162)

基因 ID

3601  [NCBI]&3600  [NCBI]

中文名
重组人 (Biotinylated, HEK293, His) 白介素-15 受体 alpha & 白介素-15融合蛋白
同用名
IL-15 R alpha; CD215; IL15RA; IL-15RA; IL-15R-alpha1; Interleukin-15; IL-15; IL15
分子量

25-28 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE

性状

Solution.

组分

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
参考文献

IL-15R alpha & IL-15 Protein, Human (Biotinylated, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IL-15R alpha & IL-15 Protein, Human (Biotinylated, HEK293, His)
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HY-P77700
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