IL-15R alpha 蛋白, Human (142a.a, HEK293, Fc)

IL-15R alpha is a high affinity receptor for IL-15 (K_d: 100 pM)^[1]. IL-15R alpha binds IL-15 and thereby activating the antitumor functions of NK cells and CD8+ T cells^[2]. IL-15R alpha plays an important role in memory CD8+ T cell homeostasis and lymphocyte development^[3]. IL-15R alpha Protein, Human (142a.a, HEK293, Fc) is a recombinant human extracellular region of IL-15R alpha (I31-T172) with a C-Terminal Fc tag, which is produced in HEK293 cells.

IL-15R α 在多种细胞类型上表达，包括淋巴细胞、骨髓细胞、非淋巴细胞和非造血细胞^[4]。IL-15R α 在 Epstein-Barr 病毒相关性胃癌 (EBVaGC) 中通过启动子高甲基化下调^[5]。
IL-15R α 的氨基酸序列在不同细胞中存在差异。物种。人 IL-15R α 与小鼠的氨基酸序列同一性<55%。
IL-15R α 是将 IL-15 从内质网转运到细胞表面以与 β (CD122) 和 γ (CD132) 结合所必需的) 响应淋巴细胞上的链^[4][6]。当与 IL-15 结合时，该复合物可延长 IL-15 的体内半衰期，并增强 NK 细胞和 CD8+ T 细胞中 IL-15 与 IL-15Rβ/γ 的结合亲和力。因此，信号传递可提高 NK 细胞和 CD8+ T 细胞的增殖和抗肿瘤活性^[2]。此外，癌细胞表面的 IL-15R α 会诱导恶性表型，例如癌细胞生长、迁移和侵袭增强，细胞凋亡减少^[5]。
IL-15R α 结合与 IL-15 结合，激活 NK 细胞和 CD8+ T 细胞的抗肿瘤功能，对记忆 CD8 T 细胞稳态和淋巴细胞发育也很重要^[2][3] 。

IL-15R alpha (mouse) exhibits a high level of IL-15 binding with high affinity when transfected to 32D-01 cells^[7].

Human

HEK293

C-hFc

Q13261-3 (I31-T172)

3601  [NCBI]

ITCPPPMSVEHADIWVKSYSLYSRERYICNSGFKRKAGTSSLTECVLNKATNVAHWTTPSLKCIKPAASSPSSNNTAATTAAIVPGSQLMPSKSPSTGTTEISSHESSHGTPSQTTAKNWELTASASHQPPGVYPQGHSDTT

60-75 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.

• [1]. Yin Guo, et al. Immunobiology of the IL-15/IL-15Rα complex as an antitumor and antiviral agent. Cytokine Growth Factor Rev. 2017 Dec;38:10-21.  [Content Brief]

  [2]. Johan Mj Van den Bergh, et al. IL-15 receptor alpha as the magic wand to boost the success of IL-15 antitumor therapies: The upswing of IL-15 transpresentation. Pharmacol Ther. 2017 Feb;170:73-79.  [Content Brief]

  [3]. Spencer W Stonier, et al. Trans-presentation: a novel mechanism regulating IL-15 delivery and responses. Immunol Lett. 2010 Jan 4;127(2):85-92.  [Content Brief]

  [4]. Patrick R Burkett, et al. IL-15R alpha expression on CD8+ T cells is dispensable for T cell memory. Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4724-9.  [Content Brief]

  [5]. Jing Wei, et al. Tumor cell-expressed IL-15Rα drives antagonistic effects on the progression and immune control of gastric cancer and is epigenetically regulated in EBV-positive gastric cancer. Cell Oncol (Dordr). 2020 Dec;43(6):1085-1097.  [Content Brief]

  [6]. Emanuela Romano, et al. Human Langerhans cells use an IL-15R-α/IL-15/pSTAT5-dependent mechanism to break T-cell tolerance against the self-differentiation tumor antigen WT1. Blood. 2012 May 31;119(22):5182-90.  [Content Brief]

  [7]. J G Giri, et al. Identification and cloning of a novel IL-15 binding protein that is structurally related to the alpha chain of the IL-2 receptor.  [Content Brief]