1. 重组蛋白
  2. Cytokines and Growth Factors CD Antigens Biotinylated Proteins
  3. Interleukin & Receptors NK Cell CD Proteins
  4. IL-15 Receptor CD215/IL-15R alpha
  5. IL-15R alpha
  6. IL-15R alpha 蛋白, Human (HEK293, His-Avi)

IL-15R alpha 蛋白, Human (HEK293, His-Avi)

目录号: HY-P78468
产品使用指南

IL-15R alpha 是 IL-15 的高亲和力受体 (Kd: 100 pM)。IL-15R alpha 与 IL-15 结合,从而激活 NK 细胞和 CD8+ T 细胞的抗肿瘤能力。IL-15R alpha 在维持记忆 CD8 T 细胞的稳态和淋巴细胞的发育中发挥着重要作用。IL-15R alpha 蛋白, Human (HEK293, His-Avi) 是一种由 HEK293 细胞产生的重组人 IL-15R alpha 的胞外片段 (I31-T205),其 C 末端带有 His 和 Avi 标签。

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50 μg   询价  
100 μg   询价  

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描述

IL-15R alpha is a high affinity receptor for IL-15 (Kd: 100 pM)[1]. IL-15R alpha binds IL-15 and thereby activating the antitumor functions of NK cells and CD8+ T cells[2]. IL-15R alpha plays an important role in memory CD8+ T cell homeostasis and lymphocyte development[3]. IL-15R alpha Protein, Human (HEK293, His-Avi) is a recombinant human extracellular region of IL-15R alpha (I31-T205) with a C-Terminal His-Avi tag, which is produced in HEK293 cells.

研究背景

IL-15R alpha is expressed on various cell types, including lymphocytes, myeloid cells, nonlymphoid and nonhematopoietic cells[4]. IL-15R alpha is down-regulated in Epstein-Barr virus associated gastric cancer (EBVaGC) via promoter hypermethylation[5].
The sequence of amino acids in IL-15R alpha differs in different species. Human IL-15R alpha shares <55% aa sequence identity with mouse.
IL-15R alpha is required for transporting of IL-15 from the endoplasmic reticulum to the cell surface to bind with β (CD122) and γ (CD132) chains on responding lymphocytes[4][6]. When binding with IL-15, the complex increases the in vivo half-life of IL-15 and enhances binding affinity of IL-15 with IL-15Rβ/γ in NK cells and CD8+ T cells. Thus, the signal transmission improves proliferation and antitumor activities of NK cells and CD8+ T cells[2]. Moreover, IL-15R alpha on the cancer cell surface induces the malignant phenotype, such as augmented cancer cell growth, migration and invasion, and decreased apoptosis[5].
IL-15R alpha binds with IL-15 and activates the antitumor functions of NK cells and CD8+ T cells, and is also important in memory CD8 T cell homeostasis and lymphocyte development[2][3].

体外研究
(In Vitro)

IL-15R alpha (mouse) exhibits a high level of IL-15 binding with high affinity when transfected to 32D-01 cells[7].

生物活性

Immobilized Human IL-15 at 1 μg/mL (100 μL/Well) on the plate. Dose response curve for Human IL-15RA His with the EC50 of 12.1 ng/mL determined by ELISA.

种属

Human

表达系统

HEK293

标签

C-Avi;C-His

蛋白编号

Q13261 (I31-T205)

中文名
重组人 (HEK293, His-Avi) 白介素-15 受体 alpha
同用名
IL-15 R alpha; CD215; IL-15RA; MGC104179
分子量

50-70 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

性状

Lyophilized powder.

组分

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5 % trehalose is added as protectant before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IL-15R alpha Protein, Human (HEK293, His-Avi)
目录号:
HY-P78468
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