1. 重组蛋白
  2. Cytokines and Growth Factors Biotinylated Proteins
  3. Interleukin & Receptors
  4. IL-17
  5. IL-17A
  6. IL-17A 蛋白, Human (Biotinylated, 132a.a, HEK293, His-Avi)

IL-17A 蛋白, Human (Biotinylated, 132a.a, HEK293, His-Avi)

目录号: HY-P78157
COA 产品使用指南

IL-17A 是一种同型二聚体细胞因子,在宿主防御机制中对许多细菌和真菌病原体以及过敏和自身免疫反应起着关键作用。IL-17A 诱导抗菌肽、细胞因子、趋化因子和基质金属蛋白酶的产生。IL-17A 蛋白, Human (Biotinylated, 132a.a, HEK293, His-Avi) 是一种生物素化的重组人 IL-17A 蛋白,在 HEK293 细胞中表达,C 末端带有 His 和Avi 标签,由 132 个氨基酸组成 (G24-A155)。

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规格 价格 是否有货 数量
20 μg ¥3350 In-stock
50 μg ¥7350 In-stock
100 μg   询价  

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描述

IL-17A is a homodimeric cytokine and plays a critical role in host defense mechanisms against many bacterial and fungal pathogens as well as allergic and autoimmune responses. IL-17A induces the production of antimicrobial peptides, cytokines, chemokines, and matrix metalloproteinases[1][2]. IL-17A Protein, Human (Biotinylated, 132a.a, HEK293, His-Avi) is a biotinylated recombinant human IL-17A protein with His tag and Avi tag at the C-terminus and is expressed in HEK293 cells. It consists of 132 amino acids (G24-A155).

研究背景

白细胞介素-17A (IL-17A),也称为 CTLA-8,属于 IL-17 细胞因子家族。IL-17A 在记忆性 Th17 细胞中表达,是记忆性 CD4+ T 细胞的产物。IL-17A 也由多种免疫细胞产生,包括 CD8+ T 细胞、γδT 细胞、自然杀伤 T (NKT) 细胞、单核细胞和中性粒细胞[1][2][3]
人IL-17A与小鼠氨基酸序列同一性为63.23%,与大鼠氨基酸序列同一性为61.90%。
IL-17A 在宿主针对许多细菌和真菌病原体的防御机制以及过敏和自身免疫反应中发挥着关键作用。IL-17A 诱导抗菌肽(防御素和 S100 蛋白)、细胞因子(IL-6、G-CSF 和 GM-CSF)、趋化因子(CXCL1、CXCL5、IL-8、CCL2 和 CCL7)和基质的产生金属蛋白酶(MMP1、MMP3 和 MMP13)。IL-17A 通过促进中性粒细胞炎症而对病毒感染有害。IL-17A 是同型二聚体细胞因子,与 IL-17F 具有相似的生物活性。IL-17A与IL-17RA以高亲和力结合,IL-17RA是IL-17A生物活性所必需的。在肿瘤发生过程中,IL-17A 会募集骨髓源性抑制细胞 (MDSC) 来抑制抗肿瘤免疫。IL-17A 还可通过诱导 IL-6[1][2] 促进体内肿瘤生长。
IL-17A 可用于研究自身免疫性疾病、感染和癌症[1][4]

体外研究
(In Vitro)

IL-17A (100 ng/mL; 24 h) inhibits the expression of thymic stromal lymphopoietin (TSLP) in human primary keratinocytes[5].
IL-17A (0.1–100 ng/mL; 0-24 h) significantly potentiates TNF-α-induced IL-8 protein secretion and gene expression in a concentration- and time-dependent manner[6].

生物活性

Immobilized Biotinylated Human IL-17A, His Tag at 2 μg/mL (100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of ≤27.0 ng/mL determined by ELISA.

种属

Human

表达系统

HEK293

标签

C-Avi;C-His

蛋白编号

Q16552 (G24-A155)

基因 ID
蛋白结构
N-term
IL-17A (G24-A155)
Accession # Q16552
His-Avi
C-term
中文名
重组人白细胞介素-17A 蛋白 (Biotinylated, 132a.a, HEK293, His-Avi)
同用名
CTLA-8; IL17; IL17A; IL-17CTLA-8; interleukin 17A
分子量

16-30 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

IL-17A Protein, Human (Biotinylated, 132a.a, HEK293, His-Avi) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IL-17A Protein, Human (Biotinylated, 132a.a, HEK293, His-Avi)
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HY-P78157
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