IL-17RD 蛋白, Mouse (CHO)

Interleukin-17 receptor D (IL-17RD) also known as Sef (similar expression to fibroblast growth factor), is a type I transmembrane protein that can regulate several signaling pathways. Within the cell, IL-17RD expression has been localized mainly to the plasma membrane. IL-17RD is a feedback inhibitor of fibroblast growth factor (FGF) signaling^[1]. IL-17RD Protein, Mouse (CHO) is produced in CHO cells, and consists of 2713 amino acids (G29-R299).

IL-17RD 是一种 I 型跨膜蛋白，在脊椎动物中进化保守，由人类染色体 3p14.3 上的单个基因座编码。在细胞内，IL-17RD 表达主要定位于质膜，尽管在高尔基体以及过表达系统中的早期和再循环核内体（核周结构）中也观察到了其表达^[1]。
人IL-17RC蛋白的氨基酸序列与小鼠IL-17C蛋白有87%的同源性。
从结构上来说，人IL-17RD由人类染色体3p14.3上的单个位点编码。mRNA由13个外显子组成，编码由26个残基的氨基末端信号肽组成的739个氨基酸的多肽产物，随后是272个氨基酸的胞外结构域、由20个氨基酸组成的短跨膜结构域和420个氨基酸的胞内结构域。领域。IL-17RD 的胞外结构域由信号肽、免疫球蛋白样结构域和纤连蛋白 III 型重复序列组成。生理学上，IL-17RD 已被证明以单体、二聚体以及寡聚体形式存在，显示出对寡聚化的偏好。在其二聚体形式中，SEF 以同二聚体以及与 FGFR1（通过其胞外、跨膜和胞内结构域）、FGFR2（通过其胞外、跨膜和胞内）、IL-17RA（主要通过其 SEFIR 结构域）的异二聚体形式存在、TNFR2（通过其胞外结构域）、TLR3（部分通过其 SEFIR 结构域）和 TLR4（部分通过其 SEFIR 结构域）以及表皮生长因子受体 (EGFR)^[1][2] 。
IL-17RD 最初被认为是斑马鱼和青蛙发育过程中 FGF 诱导的 Ras 和 MAPK 信号通路的负调节因子。随后确定它可以在包括人类在内的多种脊椎动物中调节其他受体酪氨酸激酶信号级联以及多种促炎信号传导途径，包括白介素-17A (IL17A)、Toll 样受体 (TLR) 和白细胞介素-1α (IL1α)。此外，在哺乳动物中，IL-17RD 可以抑制 ERK 和 PI3K 通路，以响应许多受体酪氨酸激酶配体。IL-17RD 是由 Toll 样受体 (TLR) 启动的 NF-κB 和 IRF 信号通路的负调节因子。IL-17RD 的 SEFIR 结构域靶向 TIR 接头、MyD88、Mal、TRIF 和 TRAM，并强烈抑制 TLR 介导的 NF-κB 激活，而 IL-17RD 缺陷导致 NF-κB 和 IRF 激活增加以及前体的上调。炎症基因。IL-17RD 可能调节 TGFβ/BMP 通路的激活。此外，IL-17RD 亚型还被证明与 HEK293T 细胞中 TAK1 的 Lys63 多泛素化相关并促进其发生。在过表达模型中，IL-17RD 通过在紫外线照射下激活 p38 MAPK 和 JNK 通路来促进细胞凋亡。IL-17RD 参与癌症、神经内分泌、炎症和免疫调节疾病的调节^[1][2]。

In collagen-induced arthritis (CIA) induction in DBA/1 mice, female DBA/1J mice (6-7 weeks old), Recombinant human IL-17RD administered intraperitoneally (5 mg/kg) every other day seven times beginning on day 22 after the first immunization. IL-17RD accelerates CIA and the inflammatory response in vivo^[3].

Measured by its ability to inhibit bFGF-induced NIH3T3 cell proliferation. The ED₅₀ this effect is 0.1396 μg/mL, corresponding to a specific activity is 7.163×10³ units/mg in the presence of 0.1 ng/mL bFGF.

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  Measured by its ability to inhibit bFGF-induced NIH3T3 cell proliferation. The ED₅₀ this effect is 0.1396 μg/mL, corresponding to a specific activity is 7.163×10³ units/mg in the presence of 0.1 ng/mL bFGF.

Mouse

CHO

Tag Free

Q8JZL1-1 (G29-R299)

171463  [NCBI]

GSGRARGADTCGWRGVGPASRNSGLHNITFRYDNCTTYLNPGGKHAIADAQNITISQYACHDQVAVTILWSPGALGIEFLKGFRVILEELKSEGRQCQQLILKDPKQLNSSFRRTGMESQPFLNMKFETDYFVKIVPFPSIKNESNYHPFFFRTRACDLLLQPDNLACKPFWKPRNLNISQHGSDMHVSFDHAPQNFGFRGFHVLYKLKHEGPFRRRTCRQDQNTETTSCLLQNVSPGDYIIELVDDSNTTRKAAQYVVKSVQSPWAGPIR

31-67 kDa

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  Greater than 95% as determined by reducing SDS-PAGE. The protein migrates as a 31-67 kDa band under reducing SDS-PAGE due to glycosylation.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg; determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Shivangi Pande, et al. Interleukin-17 receptor D (Sef) is a multi-functional regulator of cell signaling. Cell Commun Signal. 2021 Jan 12;19(1):6.  [Content Brief]

  [2]. Mark Mellett, et al. Orphan receptor IL-17RD regulates Toll-like receptor signalling via SEFIR/TIR interactions. Nat Commun. 2015 Mar 26;6:6669.  [Content Brief]

  [3]. Sihan Liu, et al. A shedding soluble form of interleukin-17 receptor D exacerbates collagen-induced arthritis through facilitating TNF-α-dependent receptor clustering. Cell Mol Immunol. 2021 Aug;18(8):1883-1895.  [Content Brief]