1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-18
  5. IL-18 蛋白, Rhesus Macaque (His)

IL-18 蛋白, Rhesus Macaque (His)

目录号: HY-P73182
COA 产品使用指南

IL-18 蛋白是一种促炎细胞因子,关键参与上皮屏障修复并调节免疫反应,特别是 Th1 细胞和 NK 细胞免疫反应。IL-18 与 IL18R1 和 IL18RAP 受体结合,形成信号三元复合物,激活 NF-κ-B,诱导炎症介质。它与 IL-12 协同作用,刺激 Th1 细胞和 NK 细胞合成 IFNG。此外,IL-18 参与细胞焦亡过程中的炎症转导,通过gasdermin-D (GSDMD) 孔释放其成熟形式。IL-18 蛋白, Rhesus Macaque (His) 是重组的 IL-18 蛋白,由 E. coli 表达,带有 N-6*His, 标签。IL-18 蛋白, Rhesus Macaque (His) 全长 192 个氨基酸,分子量约为 ~24.39 kDa。

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描述

IL-18 Protein, a pro-inflammatory cytokine, crucially participates in epithelial barrier repair and modulates immune responses, particularly in Th1 cell and NK cell immune responses. Binding to IL18R1 and IL18RAP receptors, IL-18 forms a signaling ternary complex activating NF-kappa-B, inducing inflammatory mediators. Synergizing with IL-12, it stimulates IFNG synthesis from Th1 cells and NK cells. Additionally, IL-18 is involved in transducing inflammation during pyroptosis, releasing its mature form through the gasdermin-D (GSDMD) pore. IL-18 Protein, Rhesus Macaque (His) is the recombinant Rhesus Macaque-derived IL-18 protein, expressed by E. coli, with N-6*His, labeled tag. The total length of IL-18 Protein, Rhesus Macaque (His) is 192 a.a., with molecular weight of ~24.39 kDa.

研究背景

IL-18 蛋白是一种促炎细胞因子,在上皮屏障修复和免疫反应调节中发挥着至关重要的作用,特别是在极化辅助性 T 1 (Th1) 细胞和自然杀伤 (NK) 细胞免疫反应中。IL-18 与其受体 IL18R1 和 IL18RAP 结合后,形成信号三元复合物,激活 NF-κ-B,导致炎症介质的合成。它还与 IL-12/IL-12 协同作用,诱导 Th1 细胞和 NK 细胞合成 IFNG。此外,IL-18 参与细胞焦亡下游的炎症转导,其成熟形式通过gasdermin-D (GSDMD) 孔选择性释放到细胞外空间。

生物活性

Measured by its ability to induce IFN-gamma secretion by KG-1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 for this effect is 1.095 ng/mL in the presence of 15 ng/mL TNF-alpha, corresponding to a specific activity is 9.13×10105 U/mg.

  • Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 for this effect is 1.095 ng/mL in the presence of 15 ng/mL TNF-alpha, corresponding to a specific activity is 9.13×105 U/mg.
种属

Rhesus Macaque

表达系统

E. coli

标签

N-6*His;

蛋白编号

Q9BGI5/NP_001028006.1 (A2-D193)

基因 ID

574151  [NCBI]

蛋白结构
N-term
6*His
IL-18 (A2-D193)
Accession # Q9BGI5/NP_001028006.1
C-term
同用名
Interleukin-18; IFN-gamma-inducing factor; IL-18; IL-1 gamma; Igif; IL-1F4
氨基酸序列

AAEPAEDNCINFVAMKFIDSTLYFIAEDDENLESDYFGKLESKLSIIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIINMYKDSQPRGMAVAISVKCEKISTLSCENRIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLYKLILKKKDELGDRSIMFTVQNED

分子量

Approximately 24.39 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

IL-18 Protein, Rhesus Macaque (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IL-18 Protein, Rhesus Macaque (His)
目录号:
HY-P73182
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