1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-23
  5. IL-23 alpha (175a.a) & IL-12 beta (313a.a) Heterodimer 蛋白, Mouse (HEK293, His)

IL-23 alpha (175a.a) & IL-12 beta (313a.a) Heterodimer 蛋白, Mouse (HEK293, His)

目录号: HY-P74814
COA 产品使用指南

IL-23 α 是一种关键成分,它与 IL12B 结合产生 IL-23 白细胞介素,这是一种在先天性和适应性免疫中至关重要的异二聚体细胞因子。IL-23IL-17 一起在外周组织中发挥作用,对感染做出敏锐的反应。它与 IL12RB1 和 IL23R 结合,激活 Jak-Stat 信号传导,优先刺激记忆 T 细胞,并诱导促炎细胞因子。IL-23 由抗原呈递细胞释放,在自身免疫性炎症中发挥作用,导致自身免疫性疾病和肿瘤发生,并支持 T 辅助细胞 17 细胞的扩增。IL-23 alpha (175a.a) & IL-12 beta (313a.a) Heterodimer 蛋白, Mouse (HEK293, His) 是重组的蛋白二聚体复合物,包含 IL-23 α (175a.a) 和 IL-12 β (313a.a) 异二聚体蛋白,由 HEK293 表达,带有 C-His 标签。IL-23 alpha (175a.a) & IL-12 beta (313a.a) Heterodimer 蛋白, Mouse (HEK293, His),分子量分别约为 ~47 & 45 & 26 kDa。

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规格 价格 是否有货 数量
50 μg ¥6000 In-stock
100 μg   询价  

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描述

IL-23 alpha, a crucial component, combines with IL12B to create the IL-23 interleukin, a heterodimeric cytokine pivotal in innate and adaptive immunity. Operating in peripheral tissues, IL-23, along with IL-17, responds acutely to infections. It binds to IL12RB1 and IL23R, activating Jak-Stat signaling, preferentially stimulating memory T-cells, and inducing pro-inflammatory cytokines. Released by antigen-presenting cells, IL-23 plays a role in autoimmune inflammation, contributing to autoimmune diseases and tumorigenesis, and supports the expansion of T-helper 17 cells. IL-23 alpha (175a.a) & IL-12 beta (313a.a) Heterodimer Protein, Mouse (HEK293, His) is a recombinant protein dimer complex containing mouse-derived IL-23 alpha, expressed by HEK293, with C-His labeled tag. IL-23 alpha (175a.a) & IL-12 beta (313a.a) Heterodimer Protein, Mouse (HEK293, His), has molecular weight of ~47 & 45 & 26 kDa, respectively.

研究背景

IL-23 α 作为关键成分,与 IL12B 结合形成 IL-23 白细胞介素,这是一种在先天免疫和适应性免疫中发挥关键作用的异二聚体细胞因子。IL-23IL-17 一起在外周组织中发挥作用,构成对感染的急性反应。它与由 IL12RB1 和 IL23R 组成的异二聚体受体复合物结合,激活 Jak-Stat 信号级联,优先刺激记忆 T 细胞,并促进促炎细胞因子的产生。这种细胞因子会诱导自身免疫性炎症,可能导致自身免疫性炎症性疾病,并在肿瘤发生中发挥重要作用。IL-23 由树突状细胞或巨噬细胞等抗原呈递细胞释放,与其受体复合物结合,启动信号通路,激活各种细胞反应,包括白细胞介素 17A/IL17A 的产生,并促进早期有效的细胞内细菌清除。此外,IL-23 支持辅助 T 细胞 17 细胞(一种以产生 IL-17 而闻名的 CD4 阳性辅助 T 细胞亚群)以及其他产生 IL-17 的细胞的扩增和存活。

生物活性

1.Immobilized Human IL23 Receptor hFc at 2 μg/mL (100 μLl/well) can bind Mouse IL-23 His, the EC50 of Mouse IL-23 His is 50-250 ng/mL.
2.Measured by its ability to induce IL17 secretion by mouse splenocytes and the ED50 is 0.5-4 ng/mL.

种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

Q9EQ14 (V22-A196)&P43432 (M23-S335)

基因 ID
同用名
IL-23 p19/IL-12 p40; IL23; IL-23A; Interleukin 23; SGRF
分子量

Approximately 47&45&26 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

IL-23 alpha (175a.a) & IL-12 beta (313a.a) Heterodimer Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IL-23 alpha (175a.a) & IL-12 beta (313a.a) Heterodimer Protein, Mouse (HEK293, His)
目录号:
HY-P74814
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