1. 重组蛋白
  2. Cytokines and Growth Factors CD Antigens Biotinylated Proteins
  3. Interleukin & Receptors T Cell CD Proteins B Cell CD Proteins NK Cell CD Proteins
  4. IL-2 Receptor CD25/IL-2R alpha
  5. IL-2R alpha
  6. IL-2R alpha/CD25 蛋白, Human (HEK293, His-Avi)

IL-2R alpha/CD25 蛋白, Human (HEK293, His-Avi)

目录号: HY-P78412
产品使用指南

IL-2R alpha (CD25) 是高亲和力 IL-2 受体的重要组成部分。IL-2R alpha 增强 IL-2 与其受体复合物的结合,从而调节 T 细胞生长和其他淋巴功能。IL-2R alpha/CD25 蛋白, Human (HEK293, His-Avi) 是一种重组人 IL-2R alpha 蛋白,在 HEK293 细胞中表达,由 192 个氨基酸组成 (E22-C213),C 末端带有 His 和 Avi 标签。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 是否有货
50 μg   询价  
100 μg   询价  

* Please select Quantity before adding items.

注册 MCE会员完成审核
即刻享有 积分商城 300 专属积分

Top Publications Citing Use of Products
  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

IL-2R alpha (CD25) is an essential component of high-affinity IL-2 receptors. IL-2R alpha enhances binding of IL-2 to its receptor complex so that regulates T cell growth and other lymphoid functions[1][2]. IL-2R alpha/CD25 Protein, Human (HEK293, His-Avi) is a recombinant human IL-2R alpha protein with a His tag and an Avi tag at the C-terminus and is expressed in HEK293 cells. It consists of 192 amino acids (E22-C213).

研究背景

IL-2R alpha (CD25) is a type I membrane protein. IL-2R alpha is expressed in peripheral activated T and B cells, triple-negative thymocytes, and bone marrow pre-B cells. In high tumor regulatory T (Treg) cells, IL-2R alpha is highly expressed and is a potential target for Treg deletion. The expression of IL-2R alpha is undetectable on resting T cells[1][2][3].
The sequence of amino acids in IL-2R alpha from different species is very different (less than 85% similarity among human, rat and mouse).
IL-2R alpha is an essential component of high-affinity IL-2 receptors and has no signal-transducing activity per se. IL-2R alpha functions through enhancing binding of IL-2 to its receptor complex and acts as a positive feedback regulator. IL-2 is a principal growth factor for T lymphocytes and plays an important role in T cell immune response. IL-2R alpha transcription is regulated by three positive regulatory regions (PRRs): PRRI, PRRII and PRRIII. PRRIII is an IL-2 response element[1][2].
IL-2R alpha regulates T cell growth, augments lymphocyte activation and proliferation. IL-2R alpha is involved in preventing type 1 diabetes and cancers[1][2][4].

生物活性

Immobilized Human IL-2 R alpha His at 0.2 μg/mL (100 μL/Well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody hFc with the EC50 of 15.1 ng/mL determined by ELISA.

种属

Human

表达系统

HEK293

标签

C-Avi;C-His

蛋白编号

P01589 (E22-C213)

中文名
重组人 IL-2R alpha/CD25 蛋白 (HEK293, His-Avi)
同用名
CD25; IDDM10; IL-2 R alpha; IL2R; IL2RA; p55; TCGFR
分子量

40-50 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

性状

Lyophilized powder.

组分

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5 % trehalose is added as protectant before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
IL-2R alpha/CD25 Protein, Human (HEK293, His-Avi)
目录号:
HY-P78412
需求量: