IL-3 蛋白, Rat (sf9, His)

IL-3, secreted by T-lymphocytes, mast cells, and osteoblastic cells, controls hematopoietic cell production and differentiation. It stimulates basophils, eosinophils, and monocytes, and promotes neural cell proliferation. IL-3 inhibits osteoclast differentiation and activates JAK2-STAT5 pathway. In non-hematopoietic systems, it activates PI3K/AKT and ERK pathways for cell survival under oxidative stress. IL-3 Protein, Rat (sf9, His) is the recombinant rat-derived IL-3 protein, expressed by Sf9 insect cells , with C-6*His labeled tag. The total length of IL-3 Protein, Rat (sf9, His) is 143 a.a., with molecular weight of ~24 kDa.

IL-3 是一种主要由活化的 T 淋巴细胞、肥大细胞和成骨细胞分泌的细胞因子，在控制造血祖细胞向谱系限制细胞的产生和分化中发挥着关键作用。它的影响延伸到刺激成熟的嗜碱性粒细胞、嗜酸性粒细胞和单核细胞，促进它们的功能激活。除了造血作用之外，IL-3 在神经细胞增殖和存活中也发挥着重要作用。此外，它还通过抑制破骨细胞分化来促进骨稳态，这是通过防止 NF-κ-B 核易位和激活来实现的。从机制上讲，IL-3通过由IL3RA亚基和信号转导IL3RB亚基组成的受体发挥其生物学作用。受体刺激导致 JAK2 激酶活性快速激活，从而导致 STAT5 介导的转录程序。另外，在非造血系统中，IL-3 通过激活 PI3K/AKT 和 ERK 介导的途径，有助于氧化应激下的细胞存活。

Measured in a cell proliferation assay using FDC-P1 cells and the ED₅₀ is typically 2-8 ng/mL.

Rat

Sf9 insect cells

C-6*His

P97688 (I27-C169)

24495  [NCBI]

MVLASSTTSILCMLLPLLMLFHQGLQISDRGSDAHHLLRTLDCRTIALEILVKLPYPQVSGLNNSDDKANLRNSTLRRVNLDEFLKSQEEFDSQDTTDIKSKLQKLKCCIPAAASDSVLPGVYNKDLDDFKKKLRFYVIHLKDLQPVSVSRPPQPTSSSDNFRPMTVEC

Approximately 24 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.0, 10% Glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.