IL-33 蛋白, Cynomolgus

IL-33 Protein is a cytokine belonging to the IL-1 superfamily. IL-33 induces helper T cells, mast cells, eosinophils and basophils to produce type 2 cytokines. IL-33 acts intracellularly as a nuclear factor and extracellularly as a cytokine. IL-33 mediates its biological effects via IL-1 receptor ST 2, activates NF-kappaB and MAP kinases, and drives production of T(H)2-associated cytokines from in vitro polarized T(H)2 cells. IL-33 Protein, Cynomolgus is the recombinant cynomolgus-derived IL-33 protein, expressed by E. coli , with tag free. The total length of IL-33 Protein, Cynomolgus is 159 a.a., with molecular weight of ~18.1 kDa.

白细胞介素 33 (IL-33) 是属于 IL-1 超家族的细胞因子，主要来源包括人血管树内皮细胞和小鼠外膜基质细胞、屏障组织中的上皮细胞、淋巴器官中的成纤维细胞网状细胞 (FRC)、以及神经系统中的神经胶质细胞、神经元和星形胶质细胞。IL-33 诱导辅助 T 细胞、肥大细胞、嗜酸性粒细胞和嗜碱性粒细胞产生 2 型细胞因子。IL-33 在细胞内作为核因子发挥作用，在细胞外作为细胞因子发挥作用。作为一种细胞因子，IL-33 通过 IL-1 受体 ST 2 介导其生物效应，激活 NF-kappaB 和 MAP 激酶，并驱动体外极化 T(H)2 细胞产生 T(H)2 相关细胞因子。在体内，IL-33 诱导 IL-4、IL-5 和 IL-13 的表达，导致粘膜器官发生严重的病理变化。细胞应激或暴露于炎症可以增强或诱导 IL-33 的细胞表达。IL-33 表现出多效性功能，支持 IFN-γ、IL-9 和 IL-17 主导的免疫反应，作为宿主对病原体或免疫介导的炎症性疾病反应的一部分^[1][2]。

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus IL-33 Protein is immobilized at 5 µg/mL (100 µL/well) can bind Recombinant Human IL1RL1 Protein. The ED₅₀ for this effect is 55.8 ng/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus IL-33 Protein is immobilized at 5 µg/mL (100 µL/well) can bind Recombinant Human IL1RL1 Protein. The ED₅₀ for this effect is 55.8 ng/mL.

Cynomolgus

E. coli

Tag Free

XP_005581824 (S112-I270)

102140695  [NCBI]

SITGISPITESLASLSTYNDQSITFALEDESYEIYVEDLKKDKKKDKVLLSYYESQHPSSESGDGVDGKMLMVTLSPTKDFWLQANNKEHSVELHKCEKPLPDQAFFVLHNRSFNCVSFECKTDPGVFIGVKDNHLALIKVDYSENLGSENILFKLSEI

Approximately 18.1 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.