IL-33 蛋白, Human

IL-33 Protein, Human, a Th2 cytokine, is a specific ligand for ST2L.

人 IL-33 是一种促炎蛋白，与 IL-1 细胞因子家族具有相同的结构和功能特征。它通过 IL-1RL1/ST2 受体结合并发出信号，激活 NF-kappaB 和 MAP 激酶。IL-33 诱导 Th2 细胞相关细胞因子（包括 IL-4、IL-5 和 IL-13）的产生，并发挥多种炎症相关的生物活性。IL-33-ST2 信号级联通过激活肥大细胞在慢性过敏性结膜炎的病理生理学中发挥一定作用^[1]。

1.The ED₅₀ is <0.5 ng/mL as measured by D10S cells, corresponding to a specific activity of >2.0 × 10⁶ units/mg.
2.Measured by its binding ability in a functional ELISA, mmobilized Human IL-1RL1, Fc Tag at 5 μg/mL (100 μL/well) can bind Human IL-33. The ED₅₀ for this effect is 16.45 ng/mL.

• 
  Measured by its binding ability in a functional ELISA. mmobilized Human IL-1RL1, Fc Tag at 5 μg/mL (100 μL/well) can bind Human IL-33,The ED₅₀ for this effect is 16.45 ng/mL.

Human

E. coli

Tag Free

O95760 (S112-T270)

90865  [NCBI]

SITGISPITEYLASLSTYNDQSITFALEDESYEIYVEDLKKDEKKDKVLLSYYESQHPSNESGDGVDGKMLMVTLSPTKDFWLHANNKEHSVELHKCEKPLPDQAFFVLHNMHSNCVSFECKTDPGVFIGVKDNHLALIKVDSSENLCTENILFKLSET

Approximately 18.0 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against PBS or 20 mM PB, 150 mM NaCl, pH 7.4 or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Matsuda A, et al. The role of interleukin-33 in chronic allergic conjunctivitis. Invest Ophthalmol Vis Sci. 2009 Oct;50(10):4646-52.  [Content Brief]