1. 重组蛋白
  2. Cytokines and Growth Factors CD Antigens
  3. Interleukin & Receptors Macrophage CD Proteins Epithelial cell CD Proteins Endothelial cell CD Proteins
  4. IL-4 Receptor IL-4R alpha/CD124
  5. IL-4R alpha
  6. IL-4R alpha/CD124 蛋白, Human (HEK293, Fc)

IL-4R alpha/CD124 蛋白, Human (HEK293, Fc)

目录号: HY-P70712
COA 产品使用指南

IL-4R alphaIL-4IL-13 受体共有的 α 亚基,最初发现于白细胞中。IL-4R alpha 耦连于 JAK1/2/3-STAT6 通路,参与促进 Th2 分化。IL-4R alpha/CD124, Human 由 825 个氨基酸 (M1-S825) 组成,存在跨膜结构域 (233-256 a.a) 和可溶性形式 (1-227 a.a)。可溶性 IL-4R (sIL-4R) 可抑制 IL-4 介导的细胞增殖和 T 细胞对 IL-5 的上调。IL-4R alpha/CD124, Human (M26-Q231) 在 HEK293 细胞中产生,带有 C- 末端 hFc 标记。

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50 μg ¥4600 In-stock
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描述

IL-4R alpha is a subunit alpha shared by IL-4 and IL-13 receptors, found in leukocytes originally. IL-4R alpha couples to the JAK1/2/3-STAT6 pathway and involves in promoting Th2 differentiation[1]. IL-4R alpha/CD124, Human consists of 825 amino acids (M1-S825) with a transmembrane domain (233-256 a.a) and a soluble form (1-227 a.a). Soluble IL-4R (sIL-4R) inhibits IL4-mediated cell proliferation and IL-5 up-regulation by T-cells[1]. IL-4R alpha/CD124, Human (M26-Q231) is produced in HEK293 cells with a C-Terminal hFc-tag.

研究背景

白介素 4R α (IL-4Rα) 也称为 CD124 和 B 细胞刺激因子 (BSF) 受体,是一种抗炎细胞因子,在活化的 T 细胞中高表达[1]
IL-4R α 参与不同细胞类型中两种白细胞介素受体的形成。对于I型受体,依赖于IL-4Rα结合IL-4来招募免疫细胞中的IL-2Rγ链。IL-2R γ 是多种白细胞介素受体的常见亚基,参与 IL-15 刺激中性粒细胞的吞噬作用。对于II型受体,依赖IL-4Rα结合IL-4来募集IL-13Rα1链。IL-13R α 1 是非免疫细胞中常见细胞因子受体 γ 链的替代辅助蛋白[2][3]
人类 IL-4R α 蛋白中的氨基酸与小鼠 (53.35%) 或大鼠 (52.82%) 有很大不同。
IL-4 R α 通过交替剪接或蛋白水解产生可溶形式,保持配体结合特性并抑制IL-4的生物活性。IL-4 R α 可溶性亚型 1 可以通过金属蛋白酶在细胞表面进行蛋白水解裂解(脱落)来产生[4]
IL-4 R α 在 Th2 中发挥重要作用- 免疫反应偏向、巨噬细胞活化、粘膜免疫、过敏性炎症、肿瘤进展和动脉粥样硬化[5]

体外研究
(In Vitro)

Interleukin-4Rα (IL-4Rα) shows promotion of Th2 cytokine and IgE response without hIL-4, and fails to expel N. brasiliensis worms in transgenic mice (hIL-4RαTg/mIL-4Rα-/-) infected with Nippostrongylus brasiliensis[8].

体内研究
(In Vivo)

Interleukin-4Rα (IL-4Rα) contains a immunoreceptor tyrosine-based inhibitory motifs (ITIM), plays a functional role in the regulation of IL-4-induced proliferation, ablation of ITIM results in a hyperproliferative response to IL-4 stimulation in 32D/IRS-2 cells expressing mutant IL-4R α-chains (△712 and Y713F)[6].
Recombinant sIL-4R (10 ng/mL; 3 d) inhibits IL-4-mediated proliferation and IL-5 upregulation by T cells[7].

生物活性

1.The ability to inhibit IL-4-dependent proliferation of TF1 human erythroleukemic cells has an ED50 value of 5-20 ng/mL.
2.Immobilized Human IL-4 RA-Fc at 5 μg/mL (100 μl/well) can bind Human IL-4 *: Biotinylated by NHS-biotin prior to testing.The ED50 of Human IL-4 is 2.43 ng/mL.
3.Loaded Human IL-4-His on HIS1K Biosensor, can bind Human IL-4RA-Fc with an affinity constant of 0.12 nM as determined in BLI assay.

种属

Human

表达系统

HEK293

标签

C-hFc

蛋白编号

P24394 (M26-Q231)

基因 ID
蛋白结构
N-term
IL-4Rα (M26-Q231)
Accession # P24394
hFc
C-term
中文名
重组人 (HEK293, Fc) 白介素-4 受体 alpha/CD124
同用名
Interleukin-4 receptor subunit alpha; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; CD124; IL-4-binding protein; IL4-BP; IL4R; IL4RA
氨基酸序列

MKVLQEPTCVSDYMSISTCEWKMNGPTNCSTELRLLYQLVFLLSEAHTCIPENNGGAGCVCHLLMDDVVSADNYTLDLWAGQQLLWKGSFKPSEHVKPRAPGNLTVHTNVSDTLLLTWSNPYPPDNYLYNHLTYAVNIWSENDPADFRIYNVTYLEPSLRIAASTLKSGISYRARVRAWAQCYNTTWSEWSPSTKWHNSYREPFEQ

分子量

60-70 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IL-4R alpha/CD124 Protein, Human (HEK293, Fc)
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HY-P70712
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