1. 重组蛋白
  2. Cytokines and Growth Factors CD Antigens
  3. Interleukin & Receptors Macrophage CD Proteins Epithelial cell CD Proteins Endothelial cell CD Proteins
  4. IL-4 Receptor IL-4R alpha/CD124
  5. IL-4R alpha
  6. IL-4R 蛋白, Cynomolgus (HEK293, His)

IL-4R 蛋白, Cynomolgus (HEK293, His)

目录号: HY-P77010
产品使用指南

IL-4R alpha 是 IL-4 和 IL-13 受体共有的 α 亚基,最初发现于白细胞中。IL-4R alpha 耦连于 JAK1/2/3-STAT6 通路,参与促进 Th2 分化。IL-4R alpha/CD124, Cynomolgus 由 825 个氨基酸 (M1-S825) 组成。IL-4R alpha/CD124 蛋白, Cynomolgus (M1-R232) 由 HEK293 细胞表达,包括一个 III 型纤连蛋白结构域 (125-224 a.a) 和 C- 末端 His 标记。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 是否有货
50 μg 询问价格 & 货期
100 μg 询问价格 & 货期

* Please select Quantity before adding items.

注册 MCE会员完成审核
即刻享有 积分商城 300 专属积分

Top Publications Citing Use of Products
  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

IL-4R alpha is a subunit alpha shared by IL-4 and IL-13 receptors, found in leukocytes originally. IL-4R alpha couples to the JAK1/2/3-STAT6 pathway and involves in promoting Th2 differentiation[1]. IL-4R alpha/CD124, Cynomolgus consists of 825 amino acids (M1-S825). IL-4R alpha/CD124 Protein, Cynomolgus (M1-R232) is produced in HEK293 cells, including a Fibronectin typr III domain (125-224 a.a) and a C-Terminal His-tag.

研究背景

Interleukin-4R alpha (IL-4Rα), also known as CD124 and B cell stimulatory factor (BSF) receptor, is one of the anti-inflammatory cytokines, and highly expressed in activated T-cells[1].
IL-4R alpha participates in forming two interleukin receptors in different cell types. For the type I receptor, depends on IL-4R alpha binding IL-4 to recruit IL-2R gamma chain in immune cells. IL-2R gamma is the common subunit for a variety of interleukin receptors, involved in the stimulation of neutrophil phagocytosis by IL-15. For the type II receptor, depends on IL-4R alpha binding IL-4 to recruit IL-13R alpha 1 chain. IL-13R alpha 1 is an alternat accessory protein to the common cytokine receptor gamma chain in non-immune cells[2][3].
The sequence of amino acids in IL-4R alpha proteins is very different among different animals.
IL-4 R alpha generates a soluble form by alternate splicing or proteolysis, maintaining ligand binding properties and inhibiting IL-4 bioactivity. IL-4 R alpha soluble isoform 1 can be produced by proteolytic cleavage at the cell surface (shedding) by a metalloproteinase[4].
IL-4 R alpha plays an important role in Th2-biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis[5].

体外研究
(In Vitro)

Interleukin-4Rα (IL-4Rα) shows promotion of Th2 cytokine and IgE response without hIL-4, and fails to expel N. brasiliensis worms in transgenic mice (hIL-4RαTg/mIL-4Rα-/-) infected with Nippostrongylus brasiliensis[8].

体内研究
(In Vivo)

IL-4Rα is regulated by variant IL-4 (VIL-4), produced by Mycobacterium tuberculosis and bacillus Calmette-Guérin infections of macaques, and induces expansion of Vgamma2Vdelta2 T cells[6]

生物活性

Measured by its binding ability in a functional ELISA. Immobilized IL-4 at 10 μg/mL (100 μL/well) can bind cynomolgus IL-4R and the EC50 is 50-120 ng/mL.

种属

Cynomolgus

表达系统

HEK293

标签

C-His

蛋白编号

G7Q0S7 (M1-R232)

基因 ID

/

中文名
重组猕猴 (HEK293, His) 白介素-4 受体/CD124
同用名
Interleukin-4 receptor subunit alpha; IL4R alpha; IL4R; CD124
分子量

Approximately 25.2 kDa.

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

Lyophilized powder.

组分

Lyophilized a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
IL-4R Protein, Cynomolgus (HEK293, His)
目录号:
HY-P77010
需求量: