1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-5
  5. IL-5 蛋白, Human

IL-5 蛋白, Human

目录号: HY-P7043
COA 产品使用指南

IL-5 蛋白, Human是一种选择性的人嗜酸性粒细胞功能激活剂。

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规格 价格 是否有货 数量
10 μg ¥1400 In-stock
50 μg ¥4400 In-stock
100 μg ¥6400 询价
250 μg   询价  

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  • 生物活性

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  • 参考文献

描述

IL-5 Protein, Human is a selective activator of human eosinophil function.

研究背景

Recombinant Human Interleukin-5 (rhIL-5) is a powerful and selective stimulator of human eosinophil function. IL-5 is thus the first hemopoietic factor whose elaboration in vivo can explain the selective eosinophilia and eosinophil activation seen in disease[1]. Recombinant human interleukin-5 (rhIL-5), in either liquid or semi- solid cultures, selectively induced eosinophil production from normal human bone marrow, with no activity on other cell lineages. Human interleukin-5 (IL-5) regulates the production of eosinophils in human bone marrowcultures: comparison and interaction with IL-1, IL-3, IL-6, and granulocyte-macrophage colony stimulating factor (GMCSF)[2].

生物活性

The ED50 is <1.0 ng/mL as measured by TF-1 cells, corresponding to a specific activity of >1.0 × 106 units/mg.

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

P05113 (I20-S134)

基因 ID
同用名
rHuIL-5; EDF; BCDFII; TRF
氨基酸序列

MIPTEIPTSALVKETLALLSTHRTLLIANETLRIPVPVHKNHQLCTEEIFQGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQKKKCGEERRRVNQFLDYLQEFLGVMNTEWIIES

分子量

Approximately 26.5 kDa

性状

Lyophilized powder.

组分

Lyophilized after extensive dialysis against 25 mM Tris, pH 8.0.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

IL-5 Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IL-5 Protein, Human
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HY-P7043
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