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  4. IL-6R IL-6R/CD126
  5. IL-6R alpha
  6. IL-6R 蛋白, Rat (HEK293, His)

IL-6R 蛋白, Rat (HEK293, His)

目录号: HY-P73224
产品使用指南

IL-6R alpha 是 IL-6R 的 α 亚基,也被其他白细胞介素受体共享。IL-6R alpha 作为 IL-6 激动剂,参与包括 JAK/STAT、MAPK 和 Akt 在内的信号通路。IL-6R alpha, Rat 由 462 个氨基酸 (M1-R462) 组成,存在 1 个 N 端纤维连接蛋白 III 型结构域 (215-313 a.a) 和跨膜结构 (365-385 a.a)。可溶性 IL-6R (sIL-6R) 与 SORL1 相互作用以发生内化,并结合 IL-6 和二聚体 gp130 传递反式信号。IL-6R alpha, Rat (I44-E357) 在 HEK293 细胞中产生,是可溶 IL-6R alpha 片段,并带有 C- 末端 His 标记。

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描述

IL-6R alpha is a subunit alpha of IL-6 receptors, also shared by other interleukin receptors. IL-6R alpha acts as IL-6 agonist and involves in JAK/STAT, MAPK, and Akt signaling pathway[1][2]. IL-6R alpha, Rat consists of 462 amino acids (M1-R462) with a fibronectin type-III-like domain contained in the N-terminal part (215-313 a.a) and a transmembrane domain (365-385 a.a). Soluble IL-6R (sIL-6R) interacts with SORL1 to conduct internalization and binds IL-6 and dimerized gp130 to deliver trans signalin. IL-6R alpha, Rat (I44-E357) is soluble form and produced in HEK293 cells with a C-Terminal His-tag.

研究背景

IL-6 acts as both inflammatory factor and anti-inflammatory factor, fuels cancer progression through activating a series of downstream signalling cascade including gp130 (dimers), JAK/STAT, MAPK, and Akt[1][2].
IL-6R alpha (IL-6Rα) as a part of the receptor for interleukin 6, is a type I transmembrane glycoprotein, which forms a complex with the type I transmembrane signal transducer Glycoprotein 130 (CD130) and regulates the biological activity of IL-6 with a low affinity[3].
The sequence of amino acids in IL-6R alpha proteins of rat is very different from human (54.68%), but shows high similarity with mouse (89.3%).
IL-6R alpha has 2 isoform including mIL6R (the longer one) or sIL6R (the shorter one):
The mIL6R is membrane-bound interleukin-6 receptor, has the potential to drive naive CD4+ T cells to the Th17 lineage, through 'cluster signaling' by dendritic cells[4].
The sIL-6R is soluble interleukin-6 receptor subunit, cleaved from IL-6R alpha (IL-6Rα) in activated CD4+ T cells by proteolysis, and serves as IL-6 agonist. sIL-6R binds membrane-bound IL6R and subunit IL6ST to activate regenerative and anti-inflammatory signal via IL-6 trans signaling and promotes pro-inflammatory properties of IL-6. The hydrolysis of IL-6R alpha is also called ectodomain shedding[1].
IL-6R alpha involves in regulating cell growth and differentiation, and plays an important role in regulation of immune response, acute-phase reactions and hematopoiesis[5].
However, IL-6R alpha shows tissue expression specificity in liver and some cells of the immune system, thus results a limitation of IL6 signaling[6].
It's worth noting that IL-6R alpha dysregulation is implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases, and prostate cancer[7][8].

体外研究
(In Vitro)

IL-6R alpha (sIL-6R) (100 ng/mL; 24 h) induces MMP-2 activity in rheumatoid arthritis (RA) synovial fibroblasts further 2-fold, which is involved in joint destruction in rheumatoid arthritis (RA) rats[1].
IL-6R alpha (sIL-6R) (125 ng/mL; 2-48 h) increases the mRNA level of IL-6 in primary rat osteoblasts (Ob cells), and also stimulates IL-6 xogenous added to increase the level of IL-6 mRNA in cells[2].

生物活性

Measured by its ability to bind human IL6 in functional ELISA.

种属

Rat

表达系统

HEK293

标签

C-His

蛋白编号

P22273 (M1-P364)

基因 ID

24499  [NCBI]

中文名
大鼠 IL-6R 蛋白(HEK293, His)
同用名
Interleukin-6 receptor subunit alpha; IL-6R-alpha; IL-6R 1; gp80; CD126
分子量

Approximately 39.5 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

Lyophilized powder.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IL-6R Protein, Rat (HEK293, His)
目录号:
HY-P73224
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