1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Peptide Hormone & Neuropeptides
  4. Insulin
  5. Insulin 蛋白, Human (P.pastoris)

Insulin 蛋白, Human (P.pastoris)

目录号: HY-P73243
COA 产品使用指南

胰岛素是葡萄糖调节的重要激素,通过增加细胞对单糖、氨基酸和脂肪酸的通透性来降低血糖浓度。其多种作用包括加速糖酵解、促进磷酸戊糖循环和促进肝糖原合成。胰岛素由通过二硫键连接的 A 链和 B 链组成,最近的研究强调了胰岛素对于协调代谢过程和维持葡萄糖稳态至关重要的结构特征。Insulin 蛋白, Human (P.pastoris) 是重组的蛋白二聚体复合物,包含 胰岛素蛋白,由 P. pastoris 酵母表达,不带标签。Insulin 蛋白, Human (P.pastoris),分子量约为 ~6 kDa。

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描述

Insulin, a crucial hormone for glucose regulation, reduces blood glucose concentration by increasing cellular permeability to monosaccharides, amino acids, and fatty acids. Its diverse actions encompass accelerating glycolysis, promoting the pentose phosphate cycle, and facilitating liver glycogen synthesis. Composed of A and B chains connected by disulfide bonds, recent studies highlight insulin's structural features essential for orchestrating metabolic processes and maintaining glucose homeostasis. Insulin Protein, Human (P.pastoris) is a recombinant protein dimer complex containing human-derived Insulin protein, expressed by P. pastoris, with tag free. Insulin Protein, Human (P.pastoris), has molecular weight of ~6 kDa.

研究背景

胰岛素是葡萄糖调节的关键激素,通过增强细胞对单糖、氨基酸和脂肪酸的通透性,有效降低血糖浓度。其多方面作用包括加速糖酵解、促进磷酸戊糖循环以及促进肝脏中糖原的合成。胰岛素分子本身由两个亚基组成,即 B 链和 A 链,通过两个二硫键错综复杂地连接。正如最近的研究所记录的,这些结构特征支撑了激素在协调对维持葡萄糖稳态至关重要的代谢过程中的重要作用。

生物活性

Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells and the ED50 is typically 0.2-1 µg/mL.

种属

Human

表达系统

P. pastoris

标签

Tag Free

蛋白编号

P01308 (F25-K53&G90-N110)

基因 ID
蛋白结构
N-term
Insulin (F25-K53&G90-N110)
Accession # P01308
C-term
同用名
Insulin; INS; IDDM; ILPR; IRDN; MODY10
分子量

Approximately 6 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

溶液

组分

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

Insulin Protein, Human (P.pastoris) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Insulin Protein, Human (P.pastoris)
目录号:
HY-P73243
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