1. 重组蛋白
  2. CD Antigens Receptor Proteins
  3. Epithelial cell CD Proteins Endothelial cell CD Proteins Receptor Tyrosine Kinases
  4. INSR/CD220 Insulin Receptor (INSR)
  5. Insulin R/CD220 蛋白, Human (sf9, His-GST)

Insulin R/CD220 蛋白, Human (sf9, His-GST)

目录号: HY-P73244
COA 产品使用指南

胰岛素 R/CD220 蛋白是一种受体酪氨酸激酶,通过在胰岛素结合时磷酸化 IRS 和 SHC 等底物来协调胰岛素作用。磷酸化IRS激活PI3K-AKT/PKB发挥代谢作用,激活Ras-MAPK促进生长和分化。Insulin R/CD220 蛋白, Human (sf9, His-GST) 是重组的 胰岛素 R/CD220 蛋白,由 Sf9 insect cells 表达,带有 N-His, N-GST 标签。Insulin R/CD220 蛋白, Human (sf9, His-GST) 全长 394 个氨基酸,分子量约为 ~70 kDa。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
50 μg ¥4200 In-stock
100 μg   询价  

* Please select Quantity before adding items.

注册 MCE会员完成审核
即刻享有 积分商城 300 专属积分

Top Publications Citing Use of Products
  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The insulin R/CD220 protein is a receptor tyrosine kinase that coordinates insulin action by phosphorylating substrates such as IRS and SHC upon insulin binding. Phosphorylated IRS activates PI3K-AKT/PKB to exert metabolic effects, and activates Ras-MAPK to promote growth and differentiation. Insulin R/CD220 Protein, Human (sf9, His-GST) is the recombinant human-derived Insulin R/CD220 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of Insulin R/CD220 Protein, Human (sf9, His-GST) is 394 a.a., with molecular weight of ~70 kDa.

研究背景

胰岛素 R/CD220 蛋白是一种受体酪氨酸激酶,通过胰岛素结合后多种细胞内底物的磷酸化来协调胰岛素的多种作用。这些底物包括胰岛素受体底物(IRS1、2、3、4)、SHC、GAB1、CBL 和其他信号传导中间体。磷酸化 IRS 蛋白激活两条主要信号传导途径:PI3K-AKT/PKB 途径(对于胰岛素的代谢作用至关重要)和 Ras-MAPK 途径(与 PI3K 协作调节细胞生长和分化)。PI3K-AKT/PKB 途径触发葡萄糖转运蛋白 SLC2A4/GLUT4 易位至细胞膜,促进葡萄糖转运。激活的下游效应子AKT/PKB通过磷酸化BAD诱导抗凋亡作用,调节代谢酶的表达,并调节mTORC1信号通路,整合胰岛素信号促进细胞生长和代谢。Ras/RAF/MAP2K/MAPK 通路介导胰岛素诱导的细胞生长、存活和细胞分化。除胰岛素外,该受体还可以结合胰岛素样生长因子(IGFI 和 IGFII)。由 IGF1R 和 INSR 亚型组成的混合受体对 IGF 和胰岛素表现出不同的亲和力,影响它们的激活模式。此外,在脂肪细胞中,受体抑制脂肪分解。

生物活性

The specific activity was determined to be >45 nmol/min/mg using Poly (Ala,Glu,Lys,Tyr) 6:2:5:1 as substrate.

种属

Human

表达系统

Sf9 insect cells

标签

N-His;N-GST

蛋白编号

P06213 (G989-S1382)

基因 ID
蛋白结构
N-term
His-GST
INSR (G989-S1382)
Accession # P06213
C-term
同用名
CD 220; HHF5; INSR; Insulin R; Insulin receptor; IR
分子量

Approximately 70 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 7.4, 20% gly, 0.3 mM DTT

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
Insulin R/CD220 Protein, Human (sf9, His-GST)
目录号:
HY-P73244
需求量: