1. 重组蛋白
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  3. Inhibitory Checkpoint Molecules Stem Cell CD Proteins Platelet CD Proteins Erythrocyte CD Proteins Dendritic Cell CD Proteins Epithelial cell CD Proteins Endothelial cell CD Proteins
  4. Integrin Associated Protein/CD47
  5. CD47 蛋白, Cynomolgus/Rhesus Macaque (HEK293, Fc)

CD47 蛋白, Cynomolgus/Rhesus Macaque (HEK293, Fc)

目录号: HY-P7831
COA 产品使用指南

CD47,是白细胞表面抗原,高表达的 CD47 会帮助肿瘤逃逸。CD47 抑制可导致 CD103+ 树突状细胞的激活,从而导致自然杀伤细胞的招募和激活,抑制癌症发展。CD47 蛋白, Rhesus Macaque (HEK293, Fc) 是重组的 CD47 蛋白,由 HEK293 表达,带有 C-hFc 标签。CD47 蛋白, Rhesus Macaque (HEK293, Fc) 全长 121 个氨基酸,分子量为 60-90 kDa。

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规格 价格 是否有货 数量
10 μg ¥1150 In-stock
50 μg ¥3450 In-stock
100 μg   询价  

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描述

CD47 is a leukocyte surface antigen, and high expression of CD47 helps tumors escape. CD47 inhibition leads to the activation of CD103+ dendritic cells, which leads to the recruitment and activation of natural killer cells and inhibits cancer development. CD47 Protein, Rhesus Macaque (HEK293, Fc) is the recombinant Rhesus Macaque-derived CD47 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD47 Protein, Rhesus Macaque (HEK293, Fc) is 121 a.a., with molecular weight of 60-90 kDa.

研究背景

CD47,是白细胞表面抗原 (Leukocyte surface antigen) 和血小板反应蛋白 C 端细胞结合域的受体。当细胞粘附到细胞外基质时,CD47 控制细胞内钙浓度的增加,可能在膜运输和信号转导中发挥作用。CD47 基因具有广泛的组织分布,并且在 Rh 红细胞上的表达低。高表达水平的 CD47 帮助癌细胞逃避免疫系统。如研究发现,CD47 的高表达与 HCC 患者预后较差相关。CD47 抑制,可以增强 CD103+ DCs 摄取肿瘤 DNA 的能力,从而刺激 cGAS-STING 通路,促进肝脏自然杀伤细胞在癌细胞中的浸润和活化。CD47 还结合包括血小板反应蛋白-1 (TSP-1)、信号调节蛋白 α (SIRPα)、整合素和带有 SH2 结构域的蛋白酪氨酸磷酸酶底物-1 (SHPS-1)在内的配体。而调节巨噬细胞的细胞吞噬作用、中性粒细胞的迁移以及树突状细胞、T 细胞和 B 细胞的激活。一些 CD47 抗体可能抑制 CD47-SIRPα 轴来增强癌细胞的吞噬作用。

生物活性

10 μg/mL (100 μL/well) of immobilized recombinant Rhesus Macaque Integrin-associated protein/CD47-Fc can bind Anti-Human CD47 mAb-IgG4Fc with an ED50 value of 76.1 ng/mL.

种属

Rhesus Macaque; Cynomolgus

表达系统

HEK293

标签

C-hFc

蛋白编号

F7A802/NP_001253446 (Q19-S139)

基因 ID

704980  [NCBI]

蛋白结构
N-term
CD47 (Q19-S138)
Accession # F7A802
hFc
C-term
同用名
rRhIntegrin-associated protein/CD47, Fc; Leukocyte Surface Antigen CD47; Antigenic Surface Determinant Protein OA3; Integrin-Associated Protein; IAP; Protein MER6; CD47; MER6
氨基酸序列

QLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTAPANFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFS

分子量

60-90 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM Glycine, pH 7.5.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD47 Protein, Cynomolgus/Rhesus Macaque (HEK293, Fc)
目录号:
HY-P7831
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