Intermediate capsid 蛋白 VP6, Rotavirus A (sf9, His, Myc)

The intermediate capsid protein VP6 self-assembles into an icosahedral capsid with T=13 symmetry, forming the middle layer of the virus particle. It contains 230 VP6 trimers that protect dsRNA and prevent host cell antiviral responses. Intermediate capsid protein VP6, Rotavirus A (sf9, His, myc ) is the recombinant Virus-derived Intermediate capsid protein VP6, expressed by Sf9 insect cells , with N-His, C-Myc labeled tag. The total length of Intermediate capsid protein VP6, Rotavirus A (sf9, His, myc ) is 397 a.a., with molecular weight of ~48.8 kDa.

中间衣壳蛋白VP6作为自组装组件，形成具有T=13对称性的二十面体衣壳。该衣壳由 230 个 VP6 三聚体组成，构成病毒成熟颗粒的中间同心层。在病毒复制周期中，VP6 和最里面的 VP2 衣壳在进入细胞后保持完整，从而保护 dsRNA 免遭降解并防止宿主细胞中出现不利的抗病毒反应。VP6 衣壳在双层颗粒 (DLP) 的转录活性中起着至关重要的作用，其中新生转录物在其结构范围内转录，并通过五重轴的通道挤出。VP6 与内部衣壳蛋白 VP2、外部衣壳糖蛋白 VP7 和外部衣壳蛋白 VP5* 相互作用，形成病毒衣壳整体功能的重要相互作用。

Virus

Sf9 insect cells

N-His;C-Myc

P03530 (M1-K397)

/

MEVLYSLSKTLKDARDKIVEGTLYSNVSDLIQQFNQMIVTMNGNDFQTGGIGNLPVRNWTFDFGLLGTTLLNLDANYVENARTIIEYFIDFIDNVCMDEMARESQRNGVAPQSEALRKLAGIKFKRINFDNSSEYIENWNLQNRRQRTGFVFHKPNIFPYSASFTLNRSQPMHDNLMGTMWLNAGSEIQVAGFDYSCAINAPANIQQFEHIVQLRRALTTATITLLPDAERFSFPRVINSADGATTWFFNPVILRPNNVEVEFLLNGQIINTYQARFGTIIARNFDAIRLLFQLMRPPNMTPAVNALFPQAQPFQHHATVGLTLRIESAVCESVLADANETLLANVTAVRQEYAIPVGPVFPPGMNWTELITNYSPSREDNLQRVFTVASIRSMLIK

Approximately 48.8kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.