1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. IP6K1/IHPK1 蛋白, Human (sf9, His-GST)

IP6K1/IHPK1 蛋白, Human (sf9, His-GST)

目录号: HY-P77427
产品使用指南

IP6K1,也称为 IHPK1 蛋白,通过催化肌醇六磷酸 (InsP6) 转化为肌醇二磷酸五磷酸 (InsP7/PP-InsP5),在细胞信号传导中发挥至关重要的作用。此外,该酶还参与1,3,4,5,6-五磷酸(InsP5)向PP-InsP4的转化。IP6K1/IHPK1 蛋白, Human (sf9, His-GST) 是重组的 IP6K1/IHPK1 蛋白,由 Sf9 insect cells 表达,带有 N-His, N-GST 标签。IP6K1/IHPK1 蛋白, Human (sf9, His-GST) 全长 441 个氨基酸,分子量约为 ~88 kDa。

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描述

IP6K1, also known as the IHPK1 protein, plays a crucial role in cell signaling by catalyzing the conversion of inositol hexaphosphate (InsP6) to inositol diphosphate pentaphosphate (InsP7/PP-InsP5). In addition, this enzyme is also involved in the conversion of 1,3,4,5,6-pentaphosphate (InsP5) to PP-InsP4. IP6K1/IHPK1 Protein, Human (sf9, His-GST) is the recombinant human-derived IP6K1/IHPK1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of IP6K1/IHPK1 Protein, Human (sf9, His-GST) is 441 a.a., with molecular weight of ~88 kDa.

研究背景

IP6K1/IHPK1 蛋白是一种在肌醇磷酸代谢中起着至关重要作用的酶。它将肌醇六磷酸 (InsP6) 转化为二磷酸肌醇五磷酸 (InsP7/PP-InsP5),这是合成高级肌醇多磷酸的关键步骤。此外,IP6K1/IHPK1 还可催化 1,3,4,5,6-五磷酸 (InsP5) 转化为 PP-InsP4。这些酶活性有助于肌醇磷酸信号传导的复杂调节,该信号传导参与多种细胞过程,例如细胞生长、囊泡运输和离子通道调节。InsP6 转化为 InsP7/PP-InsP5 以及 InsP5 转化为 PP-InsP4 凸显了 IP6K1/IHPK1 在调节磷酸肌醇水平中的重要性,从而影响各种细胞功能(参考:未提供)。

种属

Human

表达系统

Sf9 insect cells

标签

N-His;N-GST

蛋白编号

Q92551 (M1-Q441)

基因 ID
蛋白结构
N-term
His-GST
IP6K1 (M1-Q441)
Accession # Q92551
C-term
同用名
Inositol hexakisphosphate kinase 1; InsP6 kinase 1; IHPK1; KIAA0263
分子量

Approximately 88 kDa

纯度

Greater than 85% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

IP6K1/IHPK1 Protein, Human (sf9, His-GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IP6K1/IHPK1 Protein, Human (sf9, His-GST)
目录号:
HY-P77427
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