IRF3 蛋白, Mouse (P.pastoris, Myc, His)

The IRF3 protein is an important transcriptional regulator that coordinates type I interferon-dependent immune responses against DNA and RNA viruses. In uninfected cells, it remains inactive, but viral infection triggers phosphorylation of IKBKE and TBK1 kinases, leading to conformational changes. IRF3 Protein, Mouse (P.pastoris, Myc, His) is the recombinant mouse-derived IRF3 protein, expressed by P. pastoris , with C-Myc, N-10*His labeled tag. The total length of IRF3 Protein, Mouse (P.pastoris, Myc, His) is 419 a.a., with molecular weight of ~50.9 kDa.

IRF3 是一种关键的转录调节因子，在协调针对 DNA 和 RNA 病毒的 I 型干扰素 (IFN) 依赖性免疫反应中发挥着关键作用。它通过与启动子中的干扰素刺激反应元件结合来调节 I 型 IFN 基因（IFN-α 和 IFN-β）和 IFN 刺激基因的转录，在激活 IFN-β 基因方面表现出更大的效力。在未感染的细胞中，IRF3 在细胞质中保持非活性状态，但在病毒感染后，双链 RNA 或 Toll 样受体信号传导的识别导致其被 IKBKE 和 TBK1 激酶磷酸化。这种磷酸化会诱导构象变化，促进二聚化、核定位以及与 CREB 结合蛋白的结合，形成 dsRNA 激活因子 1 (DRAF1)。该复合物激活 I 型 IFN 和 IFN 刺激基因的转录，驱动基因诱导的早期和晚期。在没有病毒感染的情况下，IRF3 作为单体维持在自动抑制状态，并且在 TBK1 和 IKBKE 磷酸化后释放其用于 DNA 结合和二聚化以及核定位。这种磷酸化是响应病毒 RNA、胞质 DNA 或细菌脂多糖而发生的，启动涉及先天接头蛋白（MAVS、STING1 或 TICAM1）的级联反应，最终激活 IRF3，随后在细胞核中诱导 IFN。

Mouse

P. pastoris

C-Myc;N-10*His

P70671 (M1-I419)

54131  [NCBI]

METPKPRILPWLVSQLDLGQLEGVAWLDESRTRFRIPWKHGLRQDAQMADFGIFQAWAEASGAYTPGKDKPDVSTWKRNFRSALNRKEVLRLAADNSKDPYDPHKVYEFVTPGARDFVHLGASPDTNGKSSLPHSQENLPKLFDGLILGPLKDEGSSDLAIVSDPSQQLPSPNVNNFLNPAPQENPLKQLLAEEQWEFEVTAFYRGRQVFQQTLFCPGGLRLVGSTADMTLPWQPVTLPDPEGFLTDKLVKEYVGQVLKGLGNGLALWQAGQCLWAQRLGHSHAFWALGEELLPDSGRGPDGEVHKDKDGAVFDLRPFVADLIAFMEGSGHSPRYTLWFCMGEMWPQDQPWVKRLVMVKVVPTCLKELLEMAREGGASSLKTVDLHISNSQPISLTSDQYKAYLQDLVEDMDFQATGNI

Approximately 50.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.