Irisin 蛋白, Human/Mouse/Rat (HEK293, Fc)

Although the effects of the irisin protein were observed in mice, it may not have a significant role in beneficial outcomes related to muscle building or stimulating browning of white adipose tissue in humans. While previous studies in mice have implicated irisin in these processes, recent studies in humans have cast doubt on its relevance. Irisin Protein, Human/Mouse/Rat (HEK293, Fc) is the recombinant human-derived Irisin protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Irisin Protein, Human/Mouse/Rat (HEK293, Fc) is 112 a.a., with molecular weight of 45-60 kDa.

尽管在小鼠中观察到鸢尾素蛋白的作用，但它可能在与肌肉锻炼或刺激人类白色脂肪组织褐变相关的有益结果中没有显着作用。虽然之前对小鼠的研究表明鸢尾素参与了这些过程，但最近对人类的研究对其相关性提出了质疑。目前尚不清楚鸢尾素对人体是否具有类似的作用，需要进一步研究以确定其精确功能以及对人体运动引起的生理变化和代谢调节的潜在影响。

1.Measured by its ability to inhibit proliferation of A549 cells. The IC₅₀ for this effect is 0.651 ng/mL, corresponding to a specific activity is 1.5361×10⁶ units/mg.
2.Measured by its ability to induce p38 MAPK activation in 3T3 L1 mouse embryonic fibroblast adipose-like cells. 1 μg/mL of Recombinant Human Irisin can effectively induce p38 MAPK activation.

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  Measured by its ability to inhibit proliferation of A549 cells. The IC₅₀ for this effect is 0.651 ng/mL, corresponding to a specific activity is 1.5361×10⁶ units/mg.

Human

HEK293

C-hFc

Q8NAU1 (D32-E143)

252995  [NCBI]

DSPSAPVNVTVRHLKANSAVVSWDVLEDEVVIGFAISQQKKDVRMLRFIQEVNTTTRSCALWDLEEDTEYIVHVQAISIQGQSPASEPVLFKTPREAEKMASKNKDEVTMKE

Approximately 45-60 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.