Irisin 蛋白, Human/Mouse/Rat (HEK293, His)

Irisin Protein is an important cytokine secreted by muscles during exercise, which has multiple functions such as regulating metabolism and improving health. Irisin Protein, Human/Mouse/Rat (HEK293, His) is a recombinant Irisin Protein expressed by HEK293 and carrying a C-6*His tag^[1][2][3].

Irisin 是一种由骨骼肌分泌的肌因子，可促进脂肪代谢，诱导白色脂肪棕色化、参与肝脏代谢，维持糖脂稳态。此外，Irisin 还可以保护心血管、骨骼肌肉及发挥神经保护作用。Irisin 的受体主要为整合素 αV/β5，介导其在脂肪、肝脏、骨骼等组织中的作用^[1][2][3]。

Irisin 蛋白 (Human/Mouse/Rat; 100 ng/mL; 24 h) 在高糖处理的 Min6 细胞中抑制了 ROS 生成、MDA 水平，增强了 SOD 活性，并逆转了 GSDMD-N、NLRP3、cleaved caspase-1 表达的升高及 IL-1β、IL-18 分泌的增加^[4]。
Irisin 蛋白 (Human/Mouse/Rat; 0-200 ng/mL; 73 h) 在 BV2 小胶质细胞中抑制了 tau K18 诱导的 P53、P21、P16 表达和 SA-β-gal 活性，以及抑制了促炎因子 TNF-α、IL-6 的水平^[5]。

Irisin 蛋白 (Human/Mouse/Rat; 0.25 μg/kg; 腹腔注射; 8 周) 在小鼠 2 型糖尿病模型中具有改善作用，可恢复胰岛形态，增加 β 细胞数量，改善胰岛素抵抗^[4]。
Irisin 蛋白 (Human/Mouse/Rat; 250 µg/kg; 每周腹腔注射; 3 月) 在 P301S 转基因小鼠中具有神经保护的作用，可抑制小胶质细胞衰老，并改善小鼠的认知功能和突触结构^[5]。

1.Measured by its ability to inhibit proliferation of A549 cells. The IC₅₀ for this effect is 6.845 ng/mL, corresponding to a specific activity is 1.4609×10⁵ units/mg.
2.Measured by its ability to induce p38 MAPK activation in 3T3 L1 mouse embryonic fibroblast adipose-like cells. 1 μg/mL of Recombinant Human Irisin can effectively induce p38 MAPK activation.

• 
  Measured by its ability to inhibit proliferation of A549 cells. The IC₅₀ for this effect is 6.845 ng/mL , corresponding to a specific activity is 1.4609×10⁵ units/mg.

Rat; Mouse; Human

HEK293

C-6*His

Q8NAU1-1 (D32-E143)

252995  [NCBI]

Partial

DSPSAPVNVTVRHLKANSAVVSWDVLEDEVVIGFAISQQKKDVRMLRFIQEVNTTTRSCALWDLEEDTEYIVHVQAISIQGQSPASEPVLFKTPREAEKMASKNKDEVTMKE

Approximately 18-28 kDa due to the glycosylation

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Hyeonwoo Kim, et al. Irisin Mediates Effects on Bone and Fat via αV Integrin Receptors. Cell.  [Content Brief]

  [2]. Polyzos SA, et al. Irisin in metabolic diseases. Endocrine. 2018 Feb;59(2):260-274.  [Content Brief]

  [3]. Liu S, et al. Role of irisin in physiology and pathology. Front Endocrinol (Lausanne). 2022 Sep 26;13:962968.  [Content Brief]

  [4]. Li T, et al. Irisin suppresses pancreatic β cell pyroptosis in T2DM by inhibiting the NLRP3-GSDMD pathway and activating the Nrf2-TrX/TXNIP signaling axis. Diabetol Metab Syndr. 2023 Nov 22;15(1):239.  [Content Brief]

  [5]. Wang C, et al. Irisin inhibits microglial senescence via TFAM-mediated mitochondrial metabolism in a mouse model of tauopathy. Immun Ageing. 2024 May 14;21(1):30.  [Content Brief]