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  4. Irisin 蛋白, Human/Mouse/Rat (HEK293, His)

Irisin 蛋白, Human/Mouse/Rat (HEK293, His)

目录号: HY-P70664
COA 产品使用指南

Irisin 蛋白, Human/Mouse/Rat (HEK293, His)是一个带有 His 标签的 Irisin 蛋白。Irisin 是一种源自 FNDC5 基因编码的激素,可通过生热促进能量消耗。

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规格 价格 是否有货 数量
10 μg ¥1500 In-stock
50 μg ¥4500 In-stock
100 μg   询价  
250 μg   询价  

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描述

Irisin Protein, Human/Mouse/Rat (HEK293, His) is a Irisin protein labeled with a His-flag. Irisin is a hormone derived from the fibronectin type III domain-containing (FNDC5) gene that can promote energy expenditure via thermogenesis[1].

研究背景

Irisin is a recently identified hormone derived from the fibronectin type III domain-containing (FNDC5) gene that is released mainly from skeletal muscle after exercise or exposure to cold. Irisin is mainly secreted by muscle and is increased with exercise. In particular, Irisin has been shown to have beneficial effects in adipose tissues, brain and bone[2].
Recombinant Human/Mouse/Rat Irisin Protein (20-200 ng/ml; 24 hours) at low concentration has no effects on E-selectin expression. And when HUVECs are treated with high concentarion irisin (200 ng/ml), it results in soluble E-selectin concentrations similar to the levels detected in the control group treated with regular growth media[1].

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q8NAU1 (D32-E143)

基因 ID

252995  [NCBI]

同用名
Fibronectin type III domain-containing protein 5; Fibronectin type III repeat-containing protein 2; Irisin; FNDC5
氨基酸序列

DSPSAPVNVTVRHLKANSAVVSWDVLEDEVVIGFAISQQKKDVRMLRFIQEVNTTTRSCALWDLEEDTEYIVHVQAISIQGQSPASEPVLFKTPREAEKMASKNKDEVTMKE

分子量

20-28 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

Lyophilized powder.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料
参考文献

Irisin Protein, Human/Mouse/Rat (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Irisin Protein, Human/Mouse/Rat (HEK293, His)
目录号:
HY-P70664
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