Irisin 蛋白, Human/Mouse/Rat (HEK293, His)

Irisin Protein, Human/Mouse/Rat (HEK293, His) is a Irisin protein labeled with a His-flag. Irisin is a hormone derived from the fibronectin type III domain-containing (FNDC5) gene that can promote energy expenditure via thermogenesis^[1].

鸢尾素是一种最近发现的源自纤连蛋白 III 型结构域 (FNDC5) 基因的激素，该基因主要在运动或暴露于寒冷后从骨骼肌中释放。鸢尾素主要由肌肉分泌，并随着运动而增加。尤其是，鸢尾素已被证明对脂肪组织、大脑和骨骼具有有益作用^[2]。
重组人/小鼠/大鼠鸢尾素蛋白（20-200 ng/ml；24小时）在低浓度下对E-选择素表达没有影响。当 HUVEC 用高浓度鸢尾素 (200 ng/ml) 处理时，可溶性 E-选择素浓度与用常规生长培养基处理的对照组检测到的水平相似^[1]。< /br>

1.Measured by its ability to inhibit proliferation of A549 cells. The IC₅₀ for this effect is 6.845 ng/mL, corresponding to a specific activity is 1.4609×10⁵ units/mg.
2.Measured by its ability to induce p38 MAPK activation in 3T3 L1 mouse embryonic fibroblast adipose-like cells. 1 μg/mL of Recombinant Human Irisin can effectively induce p38 MAPK activation.

• 
  Measured by its ability to inhibit proliferation of A549 cells. The IC₅₀ for this effect is 6.845 ng/mL , corresponding to a specific activity is 1.4609×10⁵ units/mg.

Human

HEK293

C-6*His

Q8NAU1 (D32-E143)

252995  [NCBI]

DSPSAPVNVTVRHLKANSAVVSWDVLEDEVVIGFAISQQKKDVRMLRFIQEVNTTTRSCALWDLEEDTEYIVHVQAISIQGQSPASEPVLFKTPREAEKMASKNKDEVTMKE

Approximately 18-28 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Karan S Rana, et al. Plasma irisin is elevated in type 2 diabetes and is associated with increased E-selectin levels. Cardiovasc Diabetol  [Content Brief]

  [2]. Hyeonwoo Kim, et al. Irisin Mediates Effects on Bone and Fat via αV Integrin Receptors. Cell  [Content Brief]