ITK 蛋白, Human (sf9, GST)

ITK protein is an important tyrosine kinase that regulates adaptive immune responses by affecting the development and function of T cells and NKT cells. Upon T cell receptor (TCR) activation, ITK undergoes LCK-mediated phosphorylation, leading to its activation. ITK Protein, Human (sf9, GST) is the recombinant human-derived ITK protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of ITK Protein, Human (sf9, GST) is 269 a.a., with molecular weight of ~57.7 kDa.

ITK 蛋白是一种重要的酪氨酸激酶，通过协调传统 T 细胞和非常规 NKT 细胞的发育、功能和分化，在适应性免疫反应的调节中发挥着不可或缺的作用。抗原呈递细胞 (APC) 激活 T 细胞受体 (TCR) 后，会发生磷酸化级联反应，导致 ITK 募集到细胞膜上，靠近受刺激的 TCR 受体。随后，ITK 被 LCK 磷酸化，导致其自身磷酸化并完全激活。一旦被激活，ITK 就会磷酸化 PLCG1，从而激活该脂肪酶并启动其底物的裂解。这一过程触发钙从内质网释放到细胞质中，促进活化 T 细胞核激活剂 (NFAT) 易位到细胞核中进行转录调节。此外，ITK 磷酸化必需的衔接蛋白，例如用于激活 T 细胞/LAT 蛋白和 LCP2 的接头，招募大量信号分子，包括 VAV1，最终导致淋巴因子产生、T 细胞增殖和分化。ITK 对于 γ-δ T 细胞中 TCR 介导的钙反应至关重要，并且可能在调节未成熟 γ-δ T 细胞的 Vgamma2 阳性子集中的转录组特征中发挥作用。此外，ITK 在“Tyr-530”处磷酸化 TBX21，介导其与 GATA3 的相互作用。这种多方面的参与强调了 ITK 在塑造适应性免疫反应的各个方面的关键作用。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-GST

Q08881 (R352-L620)

3702  [NCBI]

Approximately 57.7 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as 0.22 μm filtered solution in 50mM Tris, 150mM NaCl, 25% glycerol, 0.5mM PMSF, 0.5 mM TCEP, 0.1mM EDTA, 0.5mM GSH (pH 7.5).

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.