2. 重组蛋白
  3. Enzymes & Regulators
  4. Oxidoreductases (EC 1)
  5. IVD 蛋白, Mouse (sf9, His)

IVD 蛋白, Mouse (sf9, His)

目录号: HY-P73856
产品使用指南

IVD 蛋白是亮氨酸分解代谢途径的重要组成部分，可促进异戊酰辅酶 A/3-甲基丁酰辅酶 A 转化为 3-甲基丁-2-烯酰辅酶 A。该反应充当中间步骤。此外，IVD 蛋白表现出一定的氧化饱和短链酰基辅酶 A 硫酯（如戊酰辅酶 A、己烯酰辅酶 A 和丁烯酰辅酶 A）的能力，尽管程度较低。IVD 蛋白, Mouse (sf9, His) 是重组的 IVD 蛋白，由 Sf9 昆虫细胞表达，带有 N-His 标签。IVD 蛋白, Mouse (sf9, His) 全长 394 个氨基酸，分子量约为 ~45.3 kDa。

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100 μg   询价  

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描述

IVD protein, a vital component of the leucine catabolic pathway, facilitates the transformation of isovaleryl-CoA/3-methylbutanoyl-CoA into 3-methylbut-2-enoyl-CoA. This reaction acts as an intermediate step. Moreover, IVD protein demonstrates some capacity to oxidize saturated short-chain acyl-CoA thioesters like pentanoyl-CoA, hexenoyl-CoA, and butenoyl-CoA, albeit to a lesser degree. IVD Protein, Mouse (sf9, His) is the recombinant mouse-derived IVD protein, expressed by Sf9 insect cells, with N-His labeled tag. The total length of IVD Protein, Mouse (sf9, His) is 394 a.a., with molecular weight of ~45.3 kDa.
研究背景

IVD 蛋白通过催化异戊酰辅酶 A/3-甲基丁酰辅酶 A 转化为 3-甲基丁-2-烯酰辅酶 A，在亮氨酸 (Leu) 分解代谢途径中发挥至关重要的作用。该反应充当该途径的中间步骤。此外，IVD 蛋白表现出催化其他饱和短链酰基辅酶 A 硫酯（例如戊酰辅酶 A、己烯酰辅酶 A 和丁烯酰辅酶 A）氧化的能力，尽管程度较小。
种属

Mouse
表达系统

Sf9 insect cells
标签

N-His
蛋白编号

Q9JHI5 (H31-R424)
基因 ID

56357  [NCBI]
蛋白结构
N-term
His
IVD (H31-R424)
Accession # Q9JHI5
C-term
同用名
Isovaleryl-CoA dehydrogenase, mitochondrial; IVD
分子量

Approximately 45.3 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

IVD Protein, Mouse (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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