JAM-A/CD321 蛋白, Human (HEK293, His)

The JAM-A/CD321 protein is critical for the formation of epithelial tight junctions, is present in early junction development, and recruits PARD3. Binding of the PARD6-PARD3 complex may hinder PARD3-JAM1 interaction, thereby impeding tight junction assembly. JAM-A/CD321 Protein, Human (HEK293, His) is the recombinant human-derived JAM-A/CD321 protein, expressed by HEK293 , with C-6*His labeled tag.

JAM-A/CD321 蛋白似乎参与上皮细胞紧密连接的形成。它存在于细胞连接发育的早期阶段并招募 PARD3。PARD6-PARD3 复合物的结合可能会阻止 PARD3 和 JAM1 之间的相互作用，从而阻碍紧密连接组装。该蛋白还在调节单核细胞迁移中发挥作用，这对于维持上皮屏障的完整性至关重要。它作为整合素 α-L/β-2 的配体，促进记忆 T 细胞和中性粒细胞的迁移。此外，它还参与血小板活化。在微生物感染方面，JAM-A/CD321充当哺乳动物呼肠孤病毒sigma-1的受体。

Immobilized Human JAM-A at 1 μg/mL (100 μL/well) can bind Anti-JAM-A antibody. The ED₅₀ for this effect is 3.4 ng/mL.

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  Immobilized Human JAM-A at 1 μg/mL (100 μL/well) can bind Anti-JAM-A antibody. The ED₅₀ for this effect is 3.4 ng/mL.

Human

HEK293

C-6*His

Q9Y624-1 (S28-V238)

50848  [NCBI]

Extracellular Domain

SVTVHSSEPEVRIPENNPVKLSCAYSGFSSPRVEWKFDQGDTTRLVCYNNKITASYEDRVTFLPTGITFKSVTREDTGTYTCMVSEEGGNSYGEVKVKLIVLVPPSKPTVNIPSSATIGNRAVLTCSEQDGSPPSEYTWFKDGIVMPTNPKSTRAFSNSSYVLNPTTGELVFDPLSASDTGEYSCEARNGYGTPMTSNAVRMEAVERNVGV

27-31kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 100 mM Glycine, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.