Janus kinase 2/JAK2 蛋白, Human (381aa, His)

Janus kinase 2/JAK2 Protein, Human is a recombinant human JAK2 expressed in E. coli with a His tag at the N-terminus. JAK2 is a non-receptor tyrosine kinase^[1][2].

JAK2 是非受体蛋白酪氨酸激酶 Janus 激酶 (JAK) 家族的成员，该家族包括 JAK1-3 和 TYK2。JAK 作为细胞因子受体的细胞质信号传导成分，通过细胞因子介导的转磷酸化被激活，从而导致受体磷酸化以及信号转导子和转录激活子 (STAT) 蛋白的募集和磷酸化。JAK 是非受体蛋白酪氨酸激酶，作为多种细胞因子受体的催化信号传导成分，包括白细胞介素、干扰素、生长激素、促红细胞生成素和瘦素的受体^[1][2]。

Human

E. coli

N-6*His

O60674-1 (H752-G1132)

3717  [NCBI]

KPLSALDSQRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHLKFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDLALRVDQIRDNMAG

Approximately 48.6 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.