Janus kinase 2/JAK2 蛋白, Human (His)

Janus kinase 2/JAK2 Protein, Human (His) is a recombinant human JAK2 expressed in E. coli with a His tag at the N-terminus. JAK2 is a non-receptor tyrosine kinase^[1][2].

JAK2 是非受体蛋白酪氨酸激酶 Janus 激酶 (JAK) 家族的成员，该家族包括 JAK1-3 和 TYK2。JAK 作为细胞因子受体的细胞质信号传导成分，通过细胞因子介导的转磷酸化被激活，从而导致受体磷酸化以及信号转导子和转录激活子 (STAT) 蛋白的募集和磷酸化。JAK 是非受体蛋白酪氨酸激酶，作为多种细胞因子受体的催化信号传导成分，包括白细胞介素、干扰素、生长激素、促红细胞生成素和瘦素的受体^[1][2]。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

O60674-1 (833S-1132G)

3717  [NCBI]

SGAFEDRDPTQFEERHLKFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDLALRVDQIRDNMAG

Approximately 35.1 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

溶液

Liquid from sterile 50 mM Tris-HCL, 300 mM NaCl, 200 mM arginine, pH 8.0 or 50 mM Tris-HCL, 200 mM NaCl, 200 mM arginine, 2 mM DTT, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Hubbard SR. Mechanistic Insights into Regulation of JAK2 Tyrosine Kinase. Front Endocrinol (Lausanne). 2018 Jan 5;8:361.  [Content Brief]

  [2]. Su CC, et, al. Role of the tyrosine kinase JAK2 in signal transduction by growth hormone. Pediatr Nephrol. 2000 Jul;14(7):550-7.  [Content Brief]