JTB 蛋白, Human (HEK293, Fc)

JTB proteins are critical for normal cytokinesis progression, regulate cell proliferation, and may serve as components of the chromosomal passenger complex (CPC) during mitosis. It interacts with key CPC components, affects AURKB activity, and exhibits anti-apoptotic properties, inhibiting TGFB1-induced apoptosis. JTB Protein, Human (HEK293, Fc) is the recombinant human-derived JTB protein, expressed by HEK293 , with C-mFc labeled tag. The total length of JTB Protein, Human (HEK293, Fc) is 75 a.a., with molecular weight of ~38 kDa.

JTB 蛋白对于有丝分裂过程中胞质分裂的正常进行是不可或缺的，并且在调节细胞增殖中发挥着至关重要的作用。它被认为是染色体乘客复合体 (CPC) 的潜在组成部分，而染色体乘客复合体是有丝分裂的关键调节因子，在着丝粒处具有重要功能。CPC 复合物有助于确保准确的染色体排列和分离，以及参与染色质诱导的微管稳定和纺锤体组装。JTB 与 CPC 的关键组件交互，包括 AURKA、AURKB、BIRC5 和 INCENP，可能影响 AURKB 活动。此外，JTB具有抗凋亡特性，可抑制TGFB1诱导的细胞凋亡，其过度表达与线粒体变化相关，导致线粒体肿胀和膜电位降低。这些多方面的作用凸显了 JTB 参与对有丝分裂保真度和细胞存活至关重要的复杂细胞过程。

Human

HEK293

C-mFc

O76095-1 (E31-L105)

10899  [NCBI]

EAPVQEEKLSASTSNLPCWLVEEFVVAEECSPCSNFRAKTTPECGPTGYVEKITCSSSKRNEFKSCRSALMEQRL

Approximately 39 kDa due to the glycosylation

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.