Kallikrein-14 蛋白, Mouse (His)

Kallikrein-14 protein is a serine-type endopeptidase with multiple substrate specificities and has trypsin-like and chymotrypsin-like activities. It activates/inactivates protease-activated receptors (F2R, F2RL1, F2RL3) and other kallikreins (KLK1, KLK3, KLK5, KLK11). Kallikrein-14 Protein, Mouse (His) is the recombinant mouse-derived Kallikrein-14 protein, expressed by E. coli , with N-His labeled tag. The total length of Kallikrein-14 Protein, Mouse (His) is 227 a.a., with molecular weight of ~28.5 kDa.

Kallikrein-14 是一种丝氨酸型内肽酶，具有多种底物特异性，具有胰蛋白酶样和胰凝乳蛋白酶样活性。该蛋白酶参与多种蛋白酶激活受体的激活或失活，包括 F2R、F2RL1 和 F2RL3，以及其他激肽释放酶，如 KLK1、KLK3、KLK5 和 KLK11。它通过直接裂解精蛋白 SEMG1 和 SEMG2，导致 KLK3 的激活，在精浆凝块液化中发挥至关重要的作用。此外，Kallikrein-14 通过裂解桥粒芯糖蛋白 DSG1 参与表皮脱屑，导致浅表角质细胞从皮肤表面脱落。它在肿瘤进展中的参与包括在生长、侵袭和血管生成中的作用。值得注意的是，Kallikrein-14 会受到多种丝氨酸蛋白酶抑制剂的抑制，包括 SERPINA1、SERPINC1、SERPINE1 和 SERPINF2，以及抑肽酶、大豆胰蛋白酶抑制剂和亮肽素。其自蛋白水解活性可能具有调节作用，柠檬酸盐促进该酶的激活，而锌和锰（在较小程度上）则抑制该酶的激活。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Mouse

E. coli

N-His

Q8CGR5 (24I-250N)

317653  [NCBI]

IIGGYRCVRNSQPWQVALQAGPGHRFLCGGVLLSDQWVITAAHCARPILHVALGKHNIRRWEATQQVVRVARQVPHPQYQPQAHDNDLMLLKLQKKVRLGRAVKTISVASSCASPGTPCRVSGWGTIASPIARYPTALQCVNVNIMSEQACHRAYPGIITSGMVCAGVPEGGKDSCQGDSGGPLVCGGQLQGLVSWGMERCAMPGYPGVYANLCNYHSWIQRTMQSN

Approximately 28.5 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in Tris-based buffer, 50% glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.