1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. Kallikrein-4 蛋白, Human (254a.a, HEK293, His)

Kallikrein-4 蛋白, Human (254a.a, HEK293, His)

目录号: HY-P77431
COA 产品使用指南

Kallikrein-4 蛋白对于牙釉质形成至关重要,尤其是在牙齿成熟过程中,在清除牙釉质蛋白和促进晶体基质的结构图案方面发挥着重要作用。它的参与强调了它在复杂的牙齿发育过程中的重要性,强调了它在适当的牙齿结构和功能中的作用。Kallikrein-4 蛋白, Human (254a.a, HEK293, His) 是重组的 激肽释放酶 4 蛋白,由 HEK293 表达,带有 C-His 标签。Kallikrein-4 蛋白, Human (254a.a, HEK293, His) 全长 254 个氨基酸,分子量为 30-35 kDa。

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规格 价格 是否有货 数量
50 μg ¥8500 In-stock
100 μg   询价  

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描述

Kallikrein-4 Protein is crucial in enamel formation, particularly during tooth maturation, playing a significant role in clearing enamel proteins and contributing to the structural patterning of the crystalline matrix. Its involvement underscores its importance in intricate tooth development processes, emphasizing its role in proper dental structure and function. Kallikrein-4 Protein, Human (254a.a, HEK293, His) is the recombinant human-derived Kallikrein-4 protein, expressed by HEK293 , with C-His labeled tag. The total length of Kallikrein-4 Protein, Human (254a.a, HEK293, His) is 254 a.a., with molecular weight of 30-35 kDa.

研究背景

Kallikrein-4 蛋白在牙釉质形成中发挥着重要作用,特别是在牙齿发育的成熟阶段。它对于牙釉质蛋白的清除至关重要,并有助于晶体基质的正常结构模式。这强调了该蛋白质在牙齿发育和牙釉质成熟的复杂过程中的关键参与,强调了它对于适当的牙齿结构和功能的重要性。

生物活性

Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >250 pmol/min/μg.
(Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

Q9Y5K2 (M1-S254)

基因 ID
蛋白结构
N-term
Kallikrein-4 (M1-S254)
Accession # Q9Y5K2
His
C-term
同用名
Kallikrein-4; Enamel matrix serine proteinase 1; KLK-L1; Prostase; EMSP1; PRSS17; PSTS
分子量

30-35 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Kallikrein-4 Protein, Human (254a.a, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Kallikrein-4 Protein, Human (254a.a, HEK293, His)
目录号:
HY-P77431
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