Kallikrein-8 蛋白, Mouse (P.pastoris, His)

Kallikrein-8 is a serine protease that degrades proteins such as casein and fibrinogen. It cleaves L1CAM, inducing neurite outgrowth and fasciculations. Kallikrein-8 Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived Kallikrein-8 protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of Kallikrein-8 Protein, Mouse (P.pastoris, His) is 228 a.a., with molecular weight (glycosylation form) of ~32 kDa.

Kallikrein-8 是一种丝氨酸蛋白酶，具有降解多种蛋白质的能力，包括酪蛋白、纤维蛋白原、激肽原、纤连蛋白和 IV 型胶原蛋白。此外，它会响应神经活动的增强而裂解 L1CAM，从而诱导培养的海马神经元中的神经突生长和束颤。这种蛋白酶在谢弗侧支通路中孤儿和小突触纽带的形成和成熟中起着关键作用，调节海马体中谢弗侧支的长期增强，并且对于记忆获取和突触可塑性至关重要。此外，Kallikrein-8 还参与皮肤脱屑和角质形成细胞增殖，从而促进这些过程。此外，它在脊髓损伤后发病机制的第二阶段发挥着重要作用，强调了其在神经和皮肤过程中的多种功能。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Mouse

P. pastoris

N-6*His

Q61955 (I33-D260)

259277  [NCBI]

ILEGRECIPHSQPWQAALFQGERLICGGVLVGDRWVLTAAHCKKQKYSVRLGDHSLQSRDQPEQEIQVAQSIQHPCYNNSNPEDHSHDIMLIRLQNSANLGDKVKPVQLANLCPKVGQKCIISGWGTVTSPQENFPNTLNCAEVKIYSQNKCERAYPGKITEGMVCAGSSNGADTCQGDSGGPLVCDGMLQGITSWGSDPCGKPEKPGVYTKICRYTTWIKKTMDNRD

Approximately 32 kDa. The reducing (R) protein migrates as 32 kDa in SDS-PAGE due to glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered 20 mM Tris-HCl, 0.5 M NaCl, 6%Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.