1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2) Ligases (EC 6)
  4. KARS 蛋白, Human (HEK293, His)

KARS 蛋白, Human (HEK293, His)

目录号: HY-P70840
COA 产品使用指南

KARS 蛋白通过两步反应促进氨基酸与其同源 tRNA 的附着,通过单核细胞/巨噬细胞激活诱导免疫反应。在微生物感染中,它与 HIV-1 GAG 蛋白相互作用,使选择性 tRNA(3)(Lys) 包装能够启动逆转录。KARS 蛋白, Human (HEK293, His) 是重组的 KARS 蛋白,由 HEK293 表达,带有 C-6*His 标签。KARS 蛋白, Human (HEK293, His) 全长 596 个氨基酸,分子量为 70-90 kDa。

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规格 价格 是否有货 数量
10 μg ¥1700 In-stock
50 μg ¥5100 In-stock
100 μg   询价  

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  • 技术参数

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描述

The KARS protein promotes attachment of amino acids to its cognate tRNA through a two-step reaction, inducing immune responses through monocyte/macrophage activation. In microbial infections, it interacts with the HIV-1 GAG protein to enable selective tRNA(3)(Lys) packaging to initiate reverse transcription. KARS Protein, Human (HEK293, His) is the recombinant human-derived KARS protein, expressed by HEK293 , with C-6*His labeled tag. The total length of KARS Protein, Human (HEK293, His) is 596 a.a., with molecular weight of 70-90 kDa.

研究背景

KARS 蛋白负责通过两步反应催化氨基酸与其同源 tRNA 的特异性附着:氨基酸最初被 ATP 激活形成 AA-AMP,然后转移到 tRNA 的受体末端。除了在蛋白质合成中发挥作用外,KARS 分泌时还充当信号分子,通过激活单核细胞/巨噬细胞诱导免疫反应。此外,KARS 在信号分子四磷酸二腺苷 (Ap4A) 的合成中发挥着至关重要的作用,导致 HINT1 和 MITF 之间的复合物被破坏,并随后激活 MITF 转录活性。在微生物感染的情况下,KARS 与 HIV-1 病毒 GAG 蛋白相互作用,促进 tRNA(3)(Lys) 的选择性包装,作为逆转录起始的引物。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q15046 (A2-V597)

基因 ID
蛋白结构
N-term
KARS (A2-V597)
Accession # Q15046
6*His
C-term
同用名
Lysine--tRNA Ligase; Lysyl-tRNA Synthetase; LysRS; KARS; KIAA0070
氨基酸序列

AAVQAAEVKVDGSEPKLSKNELKRRLKAEKKVAEKEAKQKELSEKQLSQATAAATNHTTDNGVGPEEESVDPNQYYKIRSQAIHQLKVNGEDPYPHKFHVDISLTDFIQKYSHLQPGDHLTDITLKVAGRIHAKRASGGKLIFYDLRGEGVKLQVMANSRNYKSEEEFIHINNKLRRGDIIGVQGNPGKTKKGELSIIPYEITLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNDFVRQKFIIRSKIITYIRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMVSGMVKHITGSYKVTYHPDGPEGQAYDVDFTPPFRRINMVEELEKALGMKLPETNLFETEETRKILDDICVAKAVECPPPRTTARLLDKLVGEFLEVTCINPTFICDHPQIMSPLAKWHRSKEGLTERFELFVMKKEICNAYTELNDPMRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRVAMFLTDSNNIKEVLLFPAMKPEDKKENVATTDTLESTTVGTSV

分子量

70-90 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 20% Glycerol, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

KARS Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
KARS Protein, Human (HEK293, His)
目录号:
HY-P70840
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