Kirrel1/NEPH1 蛋白, Human (HEK293, His)

The Kirrel1/NEPH1 protein is essential for the normal function of the glomerular filtration barrier in the kidney, maintaining stable podocyte structure through finger-like foot processes and gap septal cell-cell junctions. As a signaling molecule, it requires TEC kinase to fully activate the transcription factor AP-1. Kirrel1/NEPH1 Protein, Human (HEK293, His) is the recombinant human-derived Kirrel1/NEPH1 protein, expressed by HEK293 , with C-His labeled tag.

Kirrel1/NEPH1 蛋白对于肾脏肾小球滤过屏障的正常功能至关重要。它通过促进由称为狭缝隔膜的特殊细胞与细胞连接点连接的叉指足突，在维持稳定的足细胞结构方面发挥着至关重要的作用。该蛋白充当信号分子，需要 TEC 激酶的存在才能完全激活转录因子 AP-1。Kirrel1/NEPH1 与 TJP1/ZO-1 和 NPHS2/podocin 相互作用，其与 NPHS1/nephrin 的相互作用依赖于 Kirrel1/NEPH1 糖基化。它通过其 Ig 样结构域形成同二聚体，并且当酪氨酸磷酸化时，它与 GRB2 相互作用。

Measured by the ability of the immobilized protein to support the adhesion of MS1 mouse pancreatic islet endothelial cells. When cells are added to KIRREL coated plates (15 μg/mL, 100 μL/well), > 20% will adhere specifically after 90 minutes at 37 °C.

Human

HEK293

C-His

Q96J84-1 (M1-L493)

55243  [NCBI]

Approximately 53.5 kDa.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.