Klotho beta 蛋白, Mouse (HEK293, His)

Klotho beta protein represses cholesterol 7-alpha-hydroxylase (CYP7A1), a key enzyme in bile acid synthesis. It enhances binding between fibroblast growth factor receptors and fibroblast growth factor 21, and directly interacts with FGF19 and FGF21. This positions Klotho beta as a crucial modulator of metabolic and growth factor signaling pathways. Klotho beta Protein, Mouse (HEK293, His) is the recombinant mouse-derived Klotho beta protein, expressed by HEK293 , with C-10*His labeled tag.

Klotho beta 是一种多功能蛋白，在胆固醇 7-α-羟化酶 (CYP7A1)（胆汁酸合成中的关键酶）的转录抑制中发挥作用。虽然其糖苷酶活性可能没有活性，但 Klotho beta 通过增强成纤维细胞生长因子受体（FGFR1 和 FGFR4）与成纤维细胞生长因子 21 (FGF21) 之间的结合亲和力，展示了其分子多功能性。该蛋白还通过其 C 末端与 FGF19 和 FGF21 直接相互作用，突显其参与与代谢过程和生长因子信号传导相关的调节途径。这种复杂的相互作用网络使 Klotho beta 成为调节关键生理功能的关键角色。

Immobilized Human FGF21 Protein at 2 μg/mL (100μL/well) can bind Mouse Klotho beta. The ED₅₀ for this effect is 291.3 ng/mL.

Mouse

HEK293

C-10*His

Q99N32 (F53-L995)

83379  [NCBI]

Partial

Approximately 125-130 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.