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  3. Klotho beta 蛋白, Mouse (HEK293, His)

Klotho beta 蛋白, Mouse (HEK293, His)

目录号: HY-P78714
COA 产品使用指南 技术支持

Klotho β 蛋白抑制胆固醇 7-α-羟化酶 (CYP7A1),这是胆汁酸合成的关键酶。它增强成纤维细胞生长因子受体和成纤维细胞生长因子 21 之间的结合,并直接与 FGF19 和 FGF21 相互作用。这使得 Klotho beta 成为代谢和生长因子信号通路的重要调节剂。Klotho beta 蛋白, Mouse (HEK293, His) 是重组的 Klotho β 蛋白,由 HEK293 表达,带有 C-10*His 标签。

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5 μg ¥675 In-stock
10 μg ¥1080 In-stock
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  • 生物活性

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描述

Klotho beta protein represses cholesterol 7-alpha-hydroxylase (CYP7A1), a key enzyme in bile acid synthesis. It enhances binding between fibroblast growth factor receptors and fibroblast growth factor 21, and directly interacts with FGF19 and FGF21. This positions Klotho beta as a crucial modulator of metabolic and growth factor signaling pathways. Klotho beta Protein, Mouse (HEK293, His) is the recombinant mouse-derived Klotho beta protein, expressed by HEK293 , with C-10*His labeled tag.

研究背景

Klotho beta 是一种多功能蛋白,在胆固醇 7-α-羟化酶 (CYP7A1)(胆汁酸合成中的关键酶)的转录抑制中发挥作用。虽然其糖苷酶活性可能没有活性,但 Klotho beta 通过增强成纤维细胞生长因子受体(FGFR1 和 FGFR4)与成纤维细胞生长因子 21 (FGF21) 之间的结合亲和力,展示了其分子多功能性。该蛋白还通过其 C 末端与 FGF19 和 FGF21 直接相互作用,突显其参与与代谢过程和生长因子信号传导相关的调节途径。这种复杂的相互作用网络使 Klotho beta 成为调节关键生理功能的关键角色。

生物活性

Immobilized Human FGF21 Protein at 2 μg/mL (100μL/well) can bind Mouse Klotho beta. The ED50 for this effect is 291.3 ng/mL.

种属

Mouse

表达系统

HEK293

标签

C-10*His

蛋白编号

Q99N32 (F53-L995)

基因 ID
蛋白结构
N-term
Klotho beta (F53-L995)
Accession # Q99N32
10*His
C-term
蛋白长度

Partial

中文名
Klotho beta 蛋白
同用名
betaKlotho; beta-klotho; BKL; KLB; klotho beta like; klotho beta-like protein
预测分子量
110.3 kDa
分子量

Approximately 125-130 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

糖基化修饰
Yes
纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Klotho beta Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Klotho beta Protein, Mouse (HEK293, His)
目录号:
HY-P78714
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