KRT8 蛋白, Human (Baculovirus, His-Myc)

The KRT8 protein, in cooperation with KRT19, critically links the contractile apparatus of the striated rib muscle to dystrophin. This heterotetramer consists of two type I keratins and two type II keratins, forming a heterodimer with KRT18. KRT8 Protein, Human (Baculovirus, His-Myc) is the recombinant human-derived KRT8 protein, expressed by Sf9 insect cells , with N-10*His, C-Myc labeled tag. The total length of KRT8 Protein, Human (Baculovirus, His-Myc) is 483 a.a., with molecular weight of ~57.6 kDa.

KRT8 蛋白与 KRT19 结合，在连接横纹肌肋骨的收缩装置和肌营养不良蛋白方面发挥着至关重要的作用。该异四聚体由两个 I 型角蛋白和两个 II 型角蛋白组成，并与 KRT18 形成异二聚体。此外，KRT8 与 KRT20 结合，并在与 KRT18 形成异二聚体时与 PLEC 同工型 1C 相互作用。它与其他几种蛋白质形成复合物，包括 PNN、DMD（与 KRT19 结合时）、TCHP、APEX1、GPER1 和 EPPK1。此外，KRT8 与桥粒蛋白 PKP1 和 PKP2 相互作用。这些不同的蛋白质相互作用凸显了 KRT8 在促进各种细胞环境中细胞骨架的结构完整性和功能动态方面的多方面作用。

Human

Sf9 insect cells

N-10*His;C-Myc

P05787 (M1-K483)

3856  [NCBI]

MSIRVTQKSYKVSTSGPRAFSSRSYTSGPGSRISSSSFSRVGSSNFRGGLGGGYGGASGMGGITAVTVNQSLLSPLVLEVDPNIQAVRTQEKEQIKTLNNKFASFIDKVRFLEQQNKMLETKWSLLQQQKTARSNMDNMFESYINNLRRQLETLGQEKLKLEAELGNMQGLVEDFKNKYEDEINKRTEMENEFVLIKKDVDEAYMNKVELESRLEGLTDEINFLRQLYEEEIRELQSQISDTSVVLSMDNSRSLDMDSIIAEVKAQYEDIANRSRAEAESMYQIKYEELQSLAGKHGDDLRRTKTEISEMNRNISRLQAEIEGLKGQRASLEAAIADAEQRGELAIKDANAKLSELEAALQRAKQDMARQLREYQELMNVKLALDIEIATYRKLLEGEESRLESGMQNMSIHTKTTSGYAGGLSSAYGGLTSPGLSYSLGSSFGSGAGSSSFSRTSSSRAVVVKKIETRDGKLVSESSDVLPK

Approximately 57.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.